Interactions between neurites and somite cells: inhibition and stimulation of nerve growth in the chick embryo.
Interactions between neurites and somite cells: inhibition and stimulation of nerve growth in the chick embryo.
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神经突和体细胞之间的相互作用：抑制和刺激鸡胚神经生长。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
R. Keynes
中科院分区：
文献类型：
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作者：
C. Stern;S. Sisodiya;R. Keynes
在小鸡胚胎的神经管中出现神经过程后，它们的生长被限制在邻近体的头盖骨上。在这项研究中，我们开发了一个体外系统来模拟这些组织类型之间的相互作用。先驱者神经突在它们可以生长的基质方面显示出偏好等级。正如预期的那样，组织培养塑料不支持神经生长，但这可以通过在塑料基质上涂上胶原蛋白或聚l -赖氨酸来克服。神经嵴、颅半体和一些其他组织支持生长良好，而尾半体和尾芽间质支持生长的程度要小得多。在胚胎的低温切片和上述组织的培养细胞中评估了多种凝集素的结合模式。发现花生凝集素在体外和胚胎中都可以区分颅和尾硬膜，因为它在两种情况下都优先结合尾硬膜。这种差异在硬膜组形成时就表现出来。这些发现的意义是双重的：首先，它们表明外周神经分割过程中发生的相互作用可以在体外模拟；其次，它们代表了在培养和胚胎中都可检测到的硬核组的颅部和尾部之间的分子差异的第一个实例。
After neural processes emerge from the neural tube in the chick embryo, their growth is restricted to the cranial halves of the neighbouring somites. In this study we have developed an in vitro system to model the interactions between these tissue types. Pioneer neurites display a hierarchy of preferences in terms of the substrates they can grow on. As expected, tissue culture plastic does not support neural outgrowth, but this can be overcome by coating the plastic substrate with either collagen or poly-L-lysine. Neural crest, cranial half somite, and a number of other tissues support growth well, while caudal half somite and tail bud mesenchyme do so to a much smaller extent. The binding pattern of a variety of lectins was assessed in cryostat sections of embryos and in cultured cells of the above tissues. It was found that peanut agglutinin can discriminate between cranial and caudal sclerotome both in vitro and in the embryo, since it binds preferentially to caudal sclerotome in both cases. This difference is expressed as soon as the sclerotome forms. The significance of these findings is twofold: first, they show that the interactions that take place during peripheral neural segmentation can be modelled in vitro; second, they represent the first instance of a molecular difference between the cranial and caudal halves of the sclerotome, detectable both in culture and in the embryo.