LIPOPOLYSACCHARIDE (LPS) SIGNAL-TRANSDUCTION AND CLEARANCE - DUAL ROLES FOR LPS BINDING-PROTEIN AND MEMBRANE CD14
LIPOPOLYSACCHARIDE (LPS) SIGNAL-TRANSDUCTION AND CLEARANCE - DUAL ROLES FOR LPS BINDING-PROTEIN AND MEMBRANE CD14
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DOI：
10.1074/jbc.270.10.5320
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发表时间：
1995-03-10
影响因子：
4.8
通讯作者：
TOBIAS, PS
中科院分区：
文献类型：
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作者：
GEGNER, JA;ULEVITCH, RJ;TOBIAS, PS
在生理条件下，细胞的脂多糖（LPS）活化涉及LPS结合蛋白（LBP）和膜或可溶性CD 14。我们发现LPS与LBP和膜CD 14（mCD 14）形成三元复合物。在复合物形成之后并且不同于信号转导，LBP和LPS内化。内化可以与用抗LBP抗体18 G4和抗CD 14抗体18 E12的信号转导分开。18 G4抑制LBP与mCD 14的结合，而不阻断信号转导或LPS转移至可溶性CD 14; 18 E12抑制信号转导，而不影响LPS结合和摄取。这些数据表明，虽然LPS信号转导和LPS清除利用LBP和mCD 14，但在LPS结合mCD 14后，途径分叉。
Under physiological conditions, lipopolysaccharide (LPS) activation of cells involves the LPS binding protein (LBP) and either membrane or soluble CD14. We find LPS forms a ternary complex with LBP and membrane CD14 (mCD14). Subsequent to complex formation and distinct from signal transduction, LBP and LPS internalize, Internalization can be separated from signal transduction with the anti LBP antibody 18G4 and the anti-CD14 antibody 18E12. 18G4 inhibits LBP binding to mCD14 without blocking signal transduction or LPS transfer to soluble CD14; 18E12 inhibits signal transduction without affecting LPS binding and uptake. These data show that while LPS signal transduction and LPS clearance utilize both LBP and mCD14, the pathways bifurcate after LPS binding to mCD14.