Demonstration of a K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity in enamel-and dentin-forming tissues in the rat.
Demonstration of a K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity in enamel-and dentin-forming tissues in the rat.
复制标题
证明大鼠牙釉质和牙本质形成组织中 K 刺激和哇巴因敏感的对硝基苯基磷酸酶活性。
DOI：
10.1111/j.1600-0722.1978.tb00602.x
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
H. Mörnstad
中科院分区：
文献类型：
--
作者：
H. Mörnstad
用大鼠分泌和成熟成釉器官和牙髓组织标本研究了磷酸对硝基苯酯在中性pH下的水解。通过引入非特异性碱性磷酸酶活性的抑制剂和K+刺激和哇巴因敏感的对硝基苯基磷酸酶活性的刺激剂，可以证明后者的酶活性。这种酶活性通常被认为是钠和钾刺激的腺苷三磷酸酶的代表。K+刺激活性是镁依赖性的，对氟高度敏感。它被完全抑制由3 mM的氟化物在孵育培养基中，约1 mM产生的最大抑制的一半。在3~15 mM浓度范围内，氟化物对非依赖于K+的酶活性的抑制率为50-60%。K+刺激酶活性的高氟化物敏感性可能有助于解释牙齿组织对氟化物的敏感性。
p-Nitrophenyl phosphate hydrolysis was studied at neutral pH with tissue preparations of the rat secretory and maturation enamel organs and dental pulp. By introduction of inhibitors to nonspecific alkaline phosphatase activity and stimulants to the K+-stimulated and ouabain-sensitive p-nitrophenyl phosphatase activity, the latter enzyme activity could be demonstrated. This enzyme activity is generally held to be representative of the enzyme sodium- and potassium-stimulated adenosine triphosphatase. The K+-stimulated activity was magnesium dependent and highly sensitive to fluoride. It was inhibited completely by 3 mM fluoride in the incubation medium and about 1 mM produced half the maximum inhibition. The K+-independent enzyme activity was inhibited 50-60% by fluoride in concentrations between 3 and 15 mM. The high fluoride sensitivity of the K+-stimulated activity may perhaps help to explain the vulnerability of dental tissues to fluoride.