High-speed device synchronization in optical microscopy with an open-source hardware control platform
High-speed device synchronization in optical microscopy with an open-source hardware control platform
复制标题
使用开源硬件控制平台实现光学显微镜中的高速设备同步
DOI：
10.1038/s41598-019-48455-z
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发表时间：
2019
影响因子：
4.6
通讯作者：
Paszek, Matthew J.
中科院分区：
文献类型：
--
作者：
Colville, Marshall J.;Park, Sangwoo;Zipfel, Warren R.;Paszek, Matthew J.
Azimuthal beam scanning eliminates the uneven excitation field arising from laser interference in through-objective total internal reflection fluorescence (TIRF) microscopy. The same principle can be applied to scanning angle interference microscopy (SAIM), where precision control of the scanned laser beam presents unique technical challenges for the builders of custom azimuthal scanning microscopes. Accurate synchronization between the instrument computer, beam scanning system and excitation source is required to collect high quality data and minimize sample damage in SAIM acquisitions. Drawing inspiration from open-source prototyping systems, like the Arduino microcontroller boards, we developed a new instrument control platform to be affordable, easily programmed, and broadly useful, but with integrated, precision analog circuitry and optimized firmware routines tailored to advanced microscopy. We show how the integration of waveform generation, multiplexed analog outputs, and native hardware triggers into a single central hub provides a versatile platform for performing fast circle-scanning acquisitions, including azimuthal scanning SAIM and multiangle TIRF. We also demonstrate how the low communication latency of our hardware platform can reduce image intensity and reconstruction artifacts arising from synchronization errors produced by software control. Our complete platform, including hardware design, firmware, API, and software, is available online for community-based development and collaboration.
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影响因子：
3.4
作者：
Daniel S. Johnson;R. Toledo-Crow;A. Mattheyses;S. Simon
通讯作者：
Daniel S. Johnson;R. Toledo-Crow;A. Mattheyses;S. Simon
100 欧元的实验室：一个 3D 可打印开源平台，用于斑马鱼、果蝇和线虫行为期间的荧光显微镜、光遗传学和精确温度控制
DOI：
--
发表时间：
2017
期刊：
bioRxiv
影响因子：
--
作者：
A. M. Chagas;L. P. Godino;Aristides B. Arrenberg;T. Baden
通讯作者：
T. Baden
影响因子：
48
作者：
Schindelin, Johannes;Arganda-Carreras, Ignacio;Frise, Erwin;Kaynig, Verena;Longair, Mark;Pietzsch, Tobias;Preibisch, Stephan;Rueden, Curtis;Saalfeld, Stephan;Schmid, Benjamin;Tinevez, Jean-Yves;White, Daniel James;Hartenstein, Volker;Eliceiri, Kevin;Tomancak, Pavel;Cardona, Albert
通讯作者：
Cardona, Albert
影响因子：
2.5
作者：
Mattheyses, Alexa L.;Shaw, Keith;Axelrod, Daniel
通讯作者：
Axelrod, Daniel
影响因子：
3.6
作者：
DINGEL, B;KAWATA, S
通讯作者：
KAWATA, S