High-throughput genotyping of Salmonella enterica serovar typhi allowing geographical assignment of Haplotypes and pathotypes within an urban district of Jakarta, Indonesia
High-throughput genotyping of Salmonella enterica serovar typhi allowing geographical assignment of Haplotypes and pathotypes within an urban district of Jakarta, Indonesia
复制标题
DOI：
10.1128/jcm.02249-07
复制
发表时间：
2008-05-01
影响因子：
9.4
通讯作者：
Dougan, Gordon
中科院分区：
文献类型：
--
作者：
Baker, Stephen;Holt, Kathryn;Dougan, Gordon
提供系统发育和基因型信息的高通量流行病学分型系统有利于在现场追踪细菌病原体。印度尼西亚伤寒沙门氏菌感染的发病率很高，并且与非典型表型特征相关，比如j和z66鞭毛抗原的表达。利用高通量基因分型平台研究基因组周围分散的已知核苷酸多态性，我们确定了与印度尼西亚相关的140株伤寒沙门氏菌分离株的单倍型。我们鉴定出在印度尼西亚流行了30多年的9种不同的伤寒沙门氏菌单倍型，其中8种在2年期间出现在雅加达的一个郊区。一种优势单倍型H59与j和z66鞭毛表达相关，代表了印度尼西亚特有的一种潜在致病型。系统发育分析表明，就伤寒沙门氏菌的起源而言，H59 z66（+），j（+）分离株出现得相对较晚，并且在地理上受到限制。这些数据证明了高通量基因分型平台在分析现场伤寒沙门氏菌种群方面的潜力。该研究还深入了解了伤寒沙门氏菌的进化，并证明了一种可交流、互联网友好且具有全球实用性的分子流行病学技术的价值。
High-throughput epidemiological typing systems that provide phylogenetic and genotypic information are beneficial for tracking bacterial pathogens in the field. The incidence of Salmonella enterica serovar Typhi infection in Indonesia is high and is associated with atypical phenotypic traits such as expression of the j and the z66 flagellum antigens. Utilizing a high-throughput genotyping platform to investigate known nucleotide polymorphisms dispersed around the genome, we determined the haplotypes of 140 serovar Typhi isolates associated with Indonesia. We identified nine distinct serovar Typhi haplotypes circulating in Indonesia for more than 30 years, with eight of these present in a single Jakarta suburb within a 2-year period. One dominant haplotype, H59, is associated with j and z66 flagellum expression, representing a potential pathotype unique to Indonesia. Phylogenetic analysis suggests that H59 z66(+), j(+) isolates emerged relatively recently in terms of the origin of serovar Typhi and are geographically restricted. These data demonstrate the potential of high-throughput genotyping platforms for analyzing serovar Typhi populations in the field. The study also provides insight into the evolution of serovar Typhi and demonstrates the value of a molecular epidemiological technique that is exchangeable, that is internet friendly, and that has global utility.