Acetate production from hydrogen and [13C]carbon dioxide by the microflora of human feces
Acetate production from hydrogen and [13C]carbon dioxide by the microflora of human feces
复制标题
人类粪便微生物群利用氢气和[13C]二氧化碳生产乙酸
DOI：
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发表时间：
1988
影响因子：
4.4
通讯作者：
M. Wolin
中科院分区：
文献类型：
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作者：
S. Lajoie;Shelton Bank;Terry L. Miller;M. Wolin
将来自人类的粪便悬浮液与13 CO2和H2孵育。悬浮液分别来自每克粪便（干重）携带10（8）和10（10）个产甲烷菌的受试者，以及来自不携带产甲烷菌的受试者。定量核磁共振光谱表明，乙酸标记的甲基和羧基的主题没有产甲烷菌和低浓度的产甲烷菌的主题的悬浮液形成。所形成的标记乙酸盐的量与基于H2利用率的测量所预期的量一致。没有标记的乙酸盐由来自具有较高浓度的产甲烷菌的受试者的悬浮液形成，并且基本上所有使用的H2都由CH 4产生来解释。来自具有较低浓度的产甲烷菌的受试者的悬浮液从H2和CO2产生甲烷和乙酸。结果表明，CO2还原为乙酸盐可能是人类结肠中微生物产生乙酸盐的主要途径，除非当非常高浓度的产甲烷菌（约1000 mg/L）存在时。10（10）/g [干重]粪便）。双标记乙酸也形成了从H2和13 CO2的粪便悬浮液从非产甲烷和中度产甲烷大鼠。
Fecal suspensions from humans were incubated with 13CO2 and H2. The suspensions were from subjects who harbored 10(8) and 10(10) methanogens per g (dry weight) of feces, respectively, and from a subject who did not harbor methanogens. Quantitative nuclear magnetic resonance spectroscopy showed that acetate labeled in both the methyl and carboxyl groups was formed by suspensions from the subject without methanogens and the subject with the lower concentrations of methanogens. The amounts of labeled acetate formed were in agreement with the amounts expected based on measurements of H2 utilization. No labeled acetate was formed by suspensions from the subject with the higher concentrations of methanogens, and essentially all of the H2 used was accounted for by CH4 production. Suspensions from the subject with lower concentrations of methanogens produced both methane and acetate from H2 and CO2. The results indicate that reduction of CO2 to acetate may be a major pathway for microbial production of acetate in the human colon except when very high concentrations of methanogens (ca. 10(10) per g [dry weight] of feces) are present. Double-labeled acetate was also formed from H2 and 13CO2 by fecal suspensions from nonmethanogenic and moderately methanogenic rats.