Surface chemistry and serum type both determine the nanoparticle-protein corona.
Surface chemistry and serum type both determine the nanoparticle-protein corona.
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DOI：
10.1016/j.jprot.2015.02.009
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发表时间：
2015-04-24
影响因子：
3.3
通讯作者：
Laganà A
中科院分区：
文献类型：
--
作者：
Pozzi D;Caracciolo G;Capriotti AL;Cavaliere C;La Barbera G;Anchordoquy TJ;Laganà A
体内纳米颗粒周围形成的蛋白冠是影响其生理反应的关键因素。操纵纳米颗粒特性的潜力，从而招募有利于输送的蛋白质和/或避免有害的蛋白质，为改善药物输送提供了令人兴奋的可能性。在这项工作中，我们使用纳米液相色谱串联质谱法对五种脂质配方在小鼠和人血浆中孵卵后的冠状结构进行了表征，希望提供数据，有助于更好地理解纳米颗粒性质和生理环境在将特定蛋白质招募到冠状结构中所起的作用。值得注意的是，我们发现脂质组成的微小变化可能会严重影响蛋白质冠的组成，这表明表面化学和脂质官能团的排列是调节脂质体-蛋白质相互作用的关键参与者。值得注意的是，我们提供的证据表明，在脂质体周围形成的蛋白冠受到生理环境（即血清类型）的强烈影响。这些结果可能表明，从动物模型到临床的新药物配方的转化必须在个案的基础上进行评估。
The protein corona that forms around nanoparticles in vivo is a critical factor that affects their physiological response. The potential to manipulate nanoparticle characteristics such that either proteins advantageous for delivery are recruited and/or detrimental proteins are avoided offers exciting possibilities for improving drug delivery. In this work, we used nanoliquid chromatography tandem mass spectrometry to characterize the corona of five lipid formulations after incubation in mouse and human plasma with the hope of providing data that may contribute to a better understanding of the role played by both the nanoparticle properties and the physiological environment in recruiting specific proteins to the corona. Notably, we showed that minor changes in the lipid composition might critically affect the protein corona composition demonstrating that the surface chemistry and arrangement of lipid functional groups are key players that regulate the liposome-protein interactions. Notably, we provided evidence that the protein corona that forms around liposomes is strongly affected by the physiological environment, i.e., the serum type. These results are likely to suggest that the translation of novel pharmaceutical formulations from animal models to the clinic must be evaluated on a case-by-case basis.
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影响因子：
2.9
作者：
Capriotti, Anna Laura;Caracciolo, Giulio;Lagana, Aldo
通讯作者：
Lagana, Aldo
影响因子：
10.8
作者：
Ishida, T;Harada, M;Kiwada, H
通讯作者：
Kiwada, H
DOI：
10.1016/s0005-2760(97)00169-0
发表时间：
1998-02-16
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM
影响因子：
--
作者：
Zelphati, O;Uyechi, LS;Szoka, FC
通讯作者：
Szoka, FC
影响因子：
15
作者：
Monopoli, Marco P.;Walczyk, Dorota;Dawson, Kenneth A.
通讯作者：
Dawson, Kenneth A.
影响因子：
6.7
作者：
Pozzi, Daniela;Colapicchioni, Valentina;Lagana, Aldo
通讯作者：
Lagana, Aldo