Proteomic analysis of cisplatin resistance in human ovarian cancer using 2-DE method
Proteomic analysis of cisplatin resistance in human ovarian cancer using 2-DE method
复制标题
2-DE 方法对人卵巢癌顺铂耐药性进行蛋白质组学分析
DOI：
10.1007/s11010-010-0648-6
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发表时间：
2011-02-01
影响因子：
4.3
通讯作者：
Tong, Aiping
中科院分区：
文献类型：
--
作者：
Gong, Fengming;Peng, Xingchen;Tong, Aiping
基于铂的化疗，如顺铂，是人类卵巢癌的主要治疗方法。然而，克服耐药性已成为癌症化疗中的重要问题。本研究应用双向电泳和电喷雾质谱技术（ESI-Q-TOF MS/MS）对卵巢癌顺铂敏感细胞系A2780 S和顺铂耐药细胞系A2780-CP的蛋白质表达差异进行分析。在鉴定为两种细胞系之间差异表达（±超过两倍，P< 0.05）的14个点中，通过ESI-Q-TOF MS/MS分析阳性鉴定了10个点（对应于10种独特蛋白质）。这些蛋白质包括衣壳糖蛋白、果糖二磷酸醛缩酶C、异质核核糖核蛋白A2/B1、推定的RNA结合蛋白3、Ran特异性GTP酶激活蛋白、泛素羧基末端水解酶同工酶L1、stathmin、ATPSH蛋白、染色体盒蛋白同源物3和磷酸甘油酸激酶1。本研究所鉴定的蛋白质将有助于揭示顺铂耐药的机制，并为进一步的研究提供线索。
Platinum-based chemotherapy, such as cisplatin, is the primary treatment for human ovarian cancer. However, overcoming drug resistance has become an important issue in cancer chemotherapy. In this study, we performed 2-DE and ESI-Q-TOF MS/MS analysis to identify differential proteins expression between cisplatin-sensitive (A2780S) and cisplatin-resistant (A2780-CP) ovarian cancer cell lines. Of the 14 spots identified as differentially expressed (±over twofold,P< 0.05) between the two cell lines, ten spots (corresponding to ten unique proteins) were positively identified by ESI-Q-TOF MS/MS analysis. These proteins include capsid glycoprotein, fructose-bisphosphate aldolase C, heterogeneous nuclear ribonucleoproteins A2/B1, putative RNA-binding protein 3, Ran-specific GTPase-activating protein, ubiquitin carboxyl-terminal hydrolase isozyme L1, stathmin, ATPSH protein, chromobox protein homolog3 and phosphoglycerate kinase 1. The proteins identified in this study would be useful in revealing the mechanisms underlying cisplatin resistance and also provide some clues for further research.