A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications
A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications
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一系列独特的可逆可切换荧光蛋白，具有适合各种应用的有益特性
DOI：
10.1073/pnas.1113770109
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发表时间：
2012-03-20
影响因子：
11.1
通讯作者：
Xu, Tao
中科院分区：
文献类型：
--
作者：
Chang, Hao;Zhang, Mingshu;Xu, Tao
可逆切换荧光蛋白（rrsfp）已经引起了新兴技术的广泛兴趣，包括重复跟踪蛋白质行为和超分辨率显微镜。在数量有限的rrsfp中，只有Dronpa由于其单体和其他有利的光化学性质而被广泛应用于大多数细胞生物学应用。在这里，我们开发了一系列具有有利光学特性的单体绿色rrsfp，例如每个开关的高光子输出，高光稳定性，宽范围的开关速率和ph依赖性，这使得它们在各种应用中具有潜在的用途。该系列的一个成员mgeo - m在所有绿色rsfp中显示出最高的光子预算和定位精度潜力。我们建议mgeo - m作为替代Dronpa的候选方案，用于动态跟踪、双色超分辨率成像和光学锁定检测等应用。
Reversibly switchable fluorescent proteins (RSFPs) have attracted widespread interest for emerging techniques including repeated tracking of protein behavior and superresolution microscopy. Among the limited number of RSFPs available, only Dronpa is widely employed for most cell biology applications due to its monomeric and other favorable photochemical properties. Here we developed a series of monomeric green RSFPs with beneficial optical characteristics such as high photon output per switch, high photostability, a broad range of switching rate, and pH-dependence, which make them potentially useful for various applications. One member of this series, mGeos-M, exhibits the highest photon budget and localization precision potential among all green RSFPs. We propose mGeos-M as a candidate to replace Dronpa for applications such as dynamic tracking, dual-color superresolution imaging, and optical lock-in detection.