PAX8: A Marker for Carcinoma of Mullerian Origin in Serous Effusions
PAX8: A Marker for Carcinoma of Mullerian Origin in Serous Effusions
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DOI：
10.1002/dc.21426
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发表时间：
2011-08-01
影响因子：
1.3
通讯作者：
Greenebaum, Ellen
中科院分区：
文献类型：
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作者：
Tong, Guo-Xia;Devaraj, Kalpana;Greenebaum, Ellen
PAX8是一种核转录因子，在正常和肿瘤组织中以细胞谱系依赖的方式表达有限。PAX8已在胚胎缪勒管、人输卵管和卵巢癌中检测到。然而，人们对其在女性生殖道其他部位的表达知之甚少。在本研究中，我们采用免疫组化（IHC）方法检测PAX8在正常子宫体和子宫颈、这些部位的恶性肿瘤以及恶性积液中的表达。我们在这里报道PAX8也在子宫内膜上皮细胞和宫颈内腺中检测到，后者的表达水平较低，但在这些区域的基质细胞中没有表达。所有子宫内膜癌（N = 52） PAX8阳性，而宫颈内膜腺癌（N = 5）和子宫平滑肌肉瘤（N = 3） PAX8阴性。卵巢和子宫内膜浆液转移癌中PAX8的检出率分别为70%（22/31）和68.8%（11/16）。除1例女性肾细胞癌和1例原发不明的癌外，未在非妇科起源癌或浆液积液中的非癌（N = 71）中检测到PAX8。此外，腹膜乳头状浆液性癌（N = 10） PAX8弥漫性阳性，提示与女性生殖道恶性肿瘤相似的缪勒氏起源。我们的研究结果表明，PAX8是缪勒氏源性癌的另一种免疫结构标记物，因此我们建议将PAX8纳入女性恶性浆液积液的评估中，特别是当缪勒氏源性肿瘤处于鉴别诊断中时。成岩作用。Cytopathol。2011;39:567 - 574。(C) 2010 Wiley-Liss, Inc。
PAX8 is a nuclear transcription factor with limited expression in normal and neoplastic tissues in a cell lineage-dependent manner. PAX8 has been detected in embryonic Mullerian ducts, human fallopian tubes, and ovarian carcinomas. However, little is known about its expression in other areas of the female genital tract. In this study, we used immunohistochemistry (IHC) to examine PAX8 expression in the normal uterine corpus and cervix, malignant tumors arising from these sites, and malignant effusions. We reported here that PAX8 was also detected in endometrial epithelial cells and endocervical glands, with a lower expression level in the latter, but not in the stromal cells of these areas. All endometrial carcinomas (N = 52) were positive for PAX8, whereas endocervical adenocarcinomas (N = 5) and uterine leiomyosarcomas (N = 3) were negative for PAX8. PAX8 was detected in 70% (22/31) and 68.8% (11/16) of metastatic carcinomas of the ovary and endometrium in serous effusions, respectively. No PAX8 was detected in carcinomas of non-gynecologic origin or noncarcinomas (N = 71) in serous effusions except in one renal-cell carcinoma and one carcinoma of unknown primary in a woman. In addition, papillary serous carcinomas of the peritoneum (N = 10) were diffusely positive for PAX8, implying a Mullerian origin similar to malignant tumors in the female genital tract. Our findings suggest that PAX8 is an additional IHC marker for carcinomas of Mullerian origin hence we recommend including PAX8 for evaluation of malignant serous effusions in women, especially when tumors of Mullerian origin are in the differential diagnosis. Diagn. Cytopathol. 2011;39:567-574. (C) 2010 Wiley-Liss, Inc.