EXTRACELLULAR MANGANESE-STIMULATED DEOXYRIBONUCLEASE AS A MARKER EVENT IN SPORULATION OF BACILLUS-SUBTILIS
EXTRACELLULAR MANGANESE-STIMULATED DEOXYRIBONUCLEASE AS A MARKER EVENT IN SPORULATION OF BACILLUS-SUBTILIS
复制标题
DOI：
10.1042/bj1720063
复制
发表时间：
1978-01-01
影响因子：
4.1
通讯作者：
MANDELSTAM, J
中科院分区：
文献类型：
--
作者：
AKRIGG, A;MANDELSTAM, J
B释放了大量Mn 2+刺激的DNA酶活性。枯草芽孢杆菌168在葡萄糖缺乏的培养基中孢子形成期间;在磷酸盐或N.蛋白质合成是必需的。两种形式的证据表明，生产的DNA酶与事件后期阶段II或孢子形成。抑制与孢子形成相关的生化事件的胸苷饥饿也抑制DNA酶的释放。一些无孢子突变体在第二阶段或更早被阻断，不能产生碱性磷酸酶（第二阶段事件），不产生酶。在第二阶段末期或以后阻断的突变体产生两种酶。在野生型菌株的孢子形成期间，DNA酶在碱性磷酸酶之后约1小时出现。DNA酶的产生可能是由一个尚未发现的第二阶段遗传位点控制的。
A considerable amount of Mn2+-stimulated DNase activity is released by B. subtilis 168 during sporulation in a glucose-deficient medium; much smaller amounts are released during starvation for phosphate or N. Protein synthesis is required. Two forms of evidence are presented that production of the DNase is associated with events late in stage II or sporulation. Thymidine starvation, which inhibits the biochemical events associated with sporulation, also inhibits release of the DNase. Several asporogenous mutants blocked at stage II or earlier and unable to produce alkaline phosphatase (a stage-II event) do not produce the enzyme. Mutants blocked towards the end of stage II or later produce both enzymes. During sporulation of the wild-type strain, the DNase appears about 1 h after alkaline phosphatase. The production of the DNase is probably controlled by a still-undiscovered stage-II genetic locus.