Dual-Fluorescence In Vitro Efferocytosis Assay.
Dual-Fluorescence In Vitro Efferocytosis Assay.
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双荧光体外胞吞作用测定。
DOI：
10.1007/978-1-0716-1924-7_18
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
YurdagulJr,Arif
中科院分区：
文献类型：
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作者：
YurdagulJr,Arif
巨噬细胞清除死细胞，称为“巨噬细胞增多症”，驱动炎症消退，限制坏死，并恢复体内平衡。红细胞增多症的缺陷导致许多疾病，特别是动脉粥样硬化。存在多种方法来测试巨噬细胞在体外的吞噬作用，但每一种都有明显的缺点。本章描述了一种改进的方法来测试骨髓来源的巨噬细胞的凋亡细胞结合和内化，利用链霉亲和素和生物素之间的高亲和力。
The clearance of dead cells by macrophages, termed “efferocytosis,” drives the resolution of inflammation, restricts necrosis, and restores homeostasis. Defects in efferocytosis contribute to many diseases, particularly atherosclerosis. Multiple methods to test efferocytosis by macrophages in vitro exist, but each has distinct disadvantages. This chapter describes an improved method to test apoptotic cell binding and internalization by bone marrow–derived macrophages that takes advantage of the high-affinity between streptavidin and biotin.