Conservation of the MORF4 related gene family:: identification of a new chromo domain subfamily and novel protein motif
Conservation of the MORF4 related gene family:: identification of a new chromo domain subfamily and novel protein motif
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DOI：
10.1016/s0378-1119(01)00372-9
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发表时间：
2001-03-21
期刊：
影响因子：
3.5
通讯作者：
Pereira-Smith, OM
中科院分区：
文献类型：
--
作者：
Bertram, MJ;Pereira-Smith, OM
人类MORF4相关基因（MRG）家族有7个成员，最近根据第4号染色体上的死亡因子（MORF4）在分配给互补组B的不朽细胞系中诱导复制性衰老的能力被鉴定出来(Bertram et al., 1999；细胞生物学杂志19,1；179 - 1485)。最初基于计算机的相似性搜索确定了人类视网膜母细胞瘤结合蛋白1 （RBP-1）、果蝇雄性特异性致死蛋白3 （Msl-3）、S、pombe改变极化蛋白13 （Alp13）和酵母NuA4 HAT复合体的组成部分酿酒酵母Eaf3p （Galarneau et al, 2000）。梅尔。细胞5号，927-937)，与人类MRG蛋白家族相似。这表明MRG家族可能存在于多个物种中，对其他同源物的分析将为该基因家族的功能和进化提供深入了解。在这里，我们报告了基于分子分析和序列相似性搜索的MRG家族成员存在于23个物种中。根据与主要人类MRC家族成员MRG15和MRGX的相似性，将新家族成员分为两组。与mrc15相似的家族成员定义了铬结构域超家族的一个新的、高度保守的分支。此外，三分之二的MRG家族成员的c端以及果蝇和人类的MSL-3蛋白的保守定义了一个新的蛋白质结构域，MRG结构域。这些结果表明，MRG家族在组蛋白乙酰化介导的染色质重塑转录调控中具有高度保守的作用。(C) 2001 Elsevier Science B.V.版权所有
The seven member, human MORF4 related gene (MRG) family was recently identified based on the ability of Mortality factor on chromosome 4 (MORF4) to induce replicative senescence in immortal cell lines assigned to complementation group B (Bertram et al., 1999, Mel. Cell Biol. 19, 1;179- 1485). Initial computer based similarity searches identified human retinoblastoma binding protein 1 (RBP-1), Drosophila melanognster male specific lethal-3 (Msl-3), S, pombe altered polarity-13 (Alp13) and S. cerevisiae Eaf3p, a component of the yeast NuA4 HAT complex (Galarneau et al,, 2000. Mel. Cell 5, 927-937), as having similarity to the human MRG protein family. This suggested that the MRG family might be found in multiple species, and analysis of other homologs would provide functional and evolutionary insights into this gene family. Here, we report that MRG family members are present in twenty-three species based on molecular assays and sequence similarity searches. The new family members were divided into two groups based on similarity to the predominant human MRC family members, MRG15 and MRGX. The family members similar to MRC 15 define a new, highly conserved subsection of the chrome domain superfamily. Additionally, conservation in the C-terminal two thirds of all the MRG family members and the Drosophila and human MSL-3 proteins defines a new protein domain, the MRG domain. These results indicate a highly conserved role for the MRG family in transcriptional regulation via chromatin remodeling by histone acetylation. (C) 2001 Elsevier Science B.V. All rights reserved.