Chapter 24 Serological and biochemical analysis of four antigens associated with small cell lung cancer
Chapter 24 Serological and biochemical analysis of four antigens associated with small cell lung cancer
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第24章 小细胞肺癌相关四种抗原的血清学和生化分析
DOI：
10.1016/s0169-5002(88)80026-6
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Toshitada Takahashi
中科院分区：
文献类型：
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作者：
R. Ueda;Takashi Takahashi;Hiroyoshi Watanabe;K. Nishida;K. Utsumi;Y. Ariyoshi;K. Ota;Y. Obata;Toshitada Takahashi
制备了4株与小细胞肺癌（SCLC）细胞表面抗原反应的IgG 1单克隆抗体（Moab），并进行了血清学分析。NE-25和NE-150 Moabs显示出几乎相同的特异性，与SCLC和其他神经和/或（神经）内分泌起源的肿瘤反应。NE-25 Moab沉淀25千道尔顿（kD）的抗原。此外，在某些细胞系上也识别出150 kD的条带。相比之下，NE-150 Moab仅沉淀出150 kD的条带。OE-130 Moab用于区分SCLC和非SCLC。该摩押沉淀了130 kD的主带和其他三个分子。PE-35单克隆抗体可检测35 kD的泛上皮抗原，并对16例SCLC和38例非SCLC进行了研究。SCLC的典型表型为NE-25（NE-150）+/PE-35+/OE-130−，而非SCLC的典型表型为NE-25（NE-150）−/PE-35+/OE-130+。这些单克隆抗体被发现是有用的研究异质性和细胞谱系的小细胞肺癌。
Four IgG1 monoclonal antibodies (Moab) reactive with cell surface antigens of small cell lung cancer (SCLC) were produced and serologically analysed. NE-25 and NE-150 Moabs showed an almost identical specificity, reacting with SCLC and other tumours of neural and/or (neuro)endocrine origin. NE-25 Moab precipitated an antigen of 25 kilodaltons (kD). In addition, a band of 150 kD was also recognized on certain cell lines. In contrast, NE-150 Moab precipitated only a band of 150kD. OE-130 Moab was produced to distinguish SCLC from non-SCLC. This Moab precipitated a main band of 130kD and three other molecules. PE-35 Moab detected a 35kD pan-epithelial antigen.Sixteen SCLC and 38 non-SCLC were studied with these 4 Moabs. Typical phenotype of SCLC was NE-25(NE-150)+/PE-35+/OE-130−, whereas that of non-SCLC was NE-25(NE-150)−/PE-35+/OE-130+. These Moabs were found to be useful for studying the heterogeneity and cell lineage of SCLC.