Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays.
Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays.
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DOI：
10.1093/infdis/jiaa507
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发表时间：
2020-10-01
期刊：
影响因子：
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通讯作者：
Hughes GL
中科院分区：
文献类型：
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作者：
Patterson EI;Prince T;Anderson ER;Casas-Sanchez A;Smith SL;Cansado-Utrilla C;Solomon T;Griffiths MJ;Acosta-Serrano Á;Turtle L;Hughes GL
科学界对2019冠状病毒病（COVID-19）大流行作出回应，迅速开展研究，以找到有效的策略来减轻这种疾病的负担。令人鼓舞的是，来自不同领域的研究人员正在共同努力实现这一目标。传染性严重急性呼吸综合征冠状病毒2型（SARS-CoV-2）的研究是在高防护实验室进行的;然而，通常希望在低防护水平下处理样本。为了便于从高防护实验室转移传染性样本，我们测试了常用的病毒检测方法，并为额外的实验准备了样本。在80°C下孵育，一系列去污剂、Trizol试剂和UV能量成功灭活了高滴度的SARS-CoV-2。甲醇和多聚甲醛孵育感染细胞也灭活了病毒。这些方案可以为其他实验室的SARS-CoV-2内部灭活提供框架，确保在较低的封闭水平下安全使用样本。SARS-CoV-2研究需要在低防护实验室中安全使用样本。热、紫外线和化学试剂可灭活溶液和细胞中的高滴度病毒。这些方法将有助于在下游程序中使用一次性感染样本。
The scientific community has responded to the coronavirus disease 2019 (COVID-19) pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is undertaken in high-containment laboratories; however, it is often desirable to work with samples at lower-containment levels. To facilitate the transfer of infectious samples from high-containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents, and UV energies were successful at inactivating a high titer of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in-house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower-containment levels. SARS-CoV-2 research requires the safe use of samples in low-containment laboratories. Heat, UV, and chemical reagents inactivated high titers of virus in solution and cells. These methods will facilitate use of once-infectious samples in downstream procedures.