Genotypic characteristics of a Mycobacterium sp isolated from yellowtail Seriola quinqueradiata and striped jack Pseudocaranx dentex in Japan
Genotypic characteristics of a Mycobacterium sp isolated from yellowtail Seriola quinqueradiata and striped jack Pseudocaranx dentex in Japan
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DOI：
10.1111/j.1348-0421.2012.00514.x
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发表时间：
2013-01-01
影响因子：
2.6
通讯作者：
Kawai, Kenji
中科院分区：
文献类型：
--
作者：
Imajoh, Masayuki;Sugiura, Hidehiro;Kawai, Kenji
在日本，从黄尾鱼（serola quinqueradiata）中分离出一种类似海洋分枝杆菌的分枝杆菌。经商业分枝杆菌DNA-DNA杂交试剂盒鉴定为海洋分枝杆菌。然而，其RNA聚合酶β亚基基因DNA的PCR限制性分析明确表明该分枝杆菌属溃疡分枝杆菌。PCR分析显示了该分支杆菌的基因型特征，除mup053基因序列缺失外，其他鱼类分支杆菌如海洋分枝杆菌菌株DL240490和DL045以及假梭氏分枝杆菌中也发现了mup053基因序列缺失。除了一个例外，该分枝杆菌sp.和假肖氏分枝杆菌具有相同的16S rRNA基因序列，这也可能是海洋分枝杆菌菌株DL240490和DL045的情况。同样，根据16S rRNA基因、ITS区域和hsp65基因序列的比较，该分枝杆菌与假梭氏分枝杆菌的亲缘关系比与溃疡分枝杆菌或海洋分枝杆菌的亲缘关系更密切。从分枝杆菌的差异区9扩增出约2000 bp的PCR产物，核苷酸序列显示插入IS2404，该序列长1366 bp。在该区域发现的新的单核苷酸多态性将该分枝杆菌与M. marinum菌株DL240490和M. pseudoshottsii区分开来。目前的发现提出了这些物种有共同祖先的可能性。需要进一步的研究来提高我们对其地理起源与遗传多样性之间关系的理解。
In Japan, a Mycobacterium marinum-like mycobacterium was isolated from the yellowtail, Seriola quinqueradiata. The species was identified as M. marinum by a commercial mycobacterial DNA-DNA hybridization kit. Nevertheless, PCR restriction analysis of the DNA of its RNA polymerase beta-subunit gene definitively showed that this Mycobacterium sp. was M. ulcerans. PCR analysis revealed the genotypic characteristics of M. ulcerans in the Mycobacterium sp., only the mup053 gene sequence being absent, as has been found previously in other piscine mycobacteria such as M. marinum strains DL240490 and DL045 and M. pseudoshottsii. With one exception, this Mycobacterium sp. and M. pseudoshottsii had identical 16S rRNA gene sequences, which is also probably true of M. marinum strains DL240490 and DL045. Similarly, according to comparisons of the 16S rRNA gene, ITS region, and hsp65 gene sequences, this Mycobacterium sp. is more closely related to M. pseudoshottsii than to M. ulcerans or M. marinum. A PCR product of approximately 2000 bp was amplified from region of difference 9 in the Mycobacterium sp. The nucleotide sequence revealed insertion of IS2404, the sequence of which is 1366 bp long. The novel single nucleotide polymorphisms identified in this region distinguished this Mycobacterium sp. from M. marinum strain DL240490 and M. pseudoshottsii. The present findings raise the possibility that these species have a common ancestor. Further studies are required to improve our understanding of the relationship between their geographical origin and genetic diversity.