Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo
Differential expression of immune-related cytokine genes in response to J group avian leukosis virus infection in vivo
复制标题
J群禽白血病病毒体内感染免疫相关细胞因子基因的差异表达
DOI：
10.1016/j.molimm.2014.11.004
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发表时间：
2015-03-01
影响因子：
3.6
通讯作者：
Gao, Yulong
中科院分区：
文献类型：
--
作者：
Gao, Yanni;Liu, Yongzhen;Gao, Yulong
J群禽白血病病毒（ALV-J）感染可导致免疫抑制，随后增加继发感染的易感性。先天免疫系统是预防细菌和病毒感染的第一道防线。细胞因子在先天免疫系统中起关键作用。本研究采用RT-qPCR技术检测了ALV-J感染后不同免疫组织（包括脾脏、法氏囊和盲肠扁桃体）中细胞因子mRNA的表达水平。结果表明，在感染组中，白细胞介素-6（IL-6）、IL-18、干扰素-α（IFN-α）和IFN-γ的表达水平在脾脏中显著增加，并在感染后（d.p.i.）9-12天达到比基线高数千倍的峰值水平。法氏囊中的水平略有增加，盲肠扁桃体中的水平无显著变化。此外，这三种细胞因子在感染组脾脏中的表达模式与该组病毒血症的模式相似。这些结果表明，脾脏在ALV-J感染与天然免疫系统的相互作用中起着重要作用。本研究有助于了解ALV-J感染鸡的天然免疫反应，并阐明ALV-J的致病机制。(C)2014爱思唯尔有限公司版权所有。
Infection with J group avian leukosis virus (ALV-J) can result in immunosuppression and subsequently increased susceptibility to secondary infection. The innate immune system is the first line defense system in prevention of further bacterial and viral infections. Cytokines play key roles in the innate immune system. In this study, we used RT-qPCR technology to test the cytokine mRNA expression levels in various immune tissues, including the spleen, bursa of fabricius and cecal tonsil, in the days following ALV-J infection. The results indicated that in the infected group, the expression levels of interleukin-6 (IL-6), IL-18, interferon-alpha (IFN-alpha) and IFN-gamma significantly increased in the spleen and reached peak levels that were thousandfolds higher than baselines at 9-12 days post-infection (d.p.i.). The levels in the bursa of fabricius slightly increased, and the levels in the cecal tonsil were not significantly altered. Moreover, the pattern of the expression of these three cytokines in the spleens of the infected group was similar to the pattern of viremia of this group. These results suggest that the spleen plays an important role in the interaction between ALV-J infection and the innate immune system. This study contributes to the understanding of innate immune responses to ALV-J infection and also elucidates the mechanisms of the pathogenicity of ALV-J in chickens. (C) 2014 Elsevier Ltd. All rights reserved.