Sub-Micrometer Au@PDA-125I Particles as Theranostic Embolism Beads for Radiosensitization and SPECT/CT Monitoring
Sub-Micrometer Au@PDA-125I Particles as Theranostic Embolism Beads for Radiosensitization and SPECT/CT Monitoring
复制标题
亚微米 Au@PDA-I-125 颗粒作为治疗诊断栓塞珠，用于放射增敏和 SPECT/CT 监测
DOI：
10.1002/adhm.201800375
复制
发表时间：
2018-08-22
影响因子：
10
通讯作者：
Zuo, Changjing
中科院分区：
文献类型：
--
作者：
Sun, Gaofeng;Wang, Tao;Zuo, Changjing
将Au纳米颗粒（3.8 +/- 0.6 nm）组装到亚微米Au颗粒（186.3 +/- 20.4 nm）上，并用粘附性聚多巴胺（PDA）作为栓塞珠（198.8 +/- 23.2 nm）覆盖。将放射性碘-125标记到Au@PDA上以引入内照射功能。对于Au@PDA-I-125的治疗效果，Au颗粒对MHCC 97 H肝癌细胞和荷瘤小鼠的辐射增敏。在细胞水平上，低剂量（5戈伊）射线照射后，金致敏放疗（RT）导致细胞内活性氧增加，细胞凋亡增加。由于内照射，RT导致的DNA双链断裂的自愈被抑制，诱导RT处理后细胞凋亡进一步增加。同样，3个周期的敏化RT导致肿瘤体积生长的有效控制，但Au@PDA-I-125在放射敏感性器官上或中没有伤害或放射性残留，包括甲状腺、心脏、肺、肝和脾。此外，从I-125发射的光子和Au元素的高X射线吸收使得珠适用于单光子发射计算机断层摄影/计算机断层摄影（SPECT/CT）成像。因此，Au@PDA-I-125作为治疗诊断栓塞微珠，既能增强体外RT的治疗效果，又能提供实时SPECT/CT监测的治疗时间窗。
Au nanoparticles (3.8 +/- 0.6 nm) are assembled to sub-micrometer Au particles (186.3 +/- 20.4 nm) and covered with adhesive polydopamine (PDA) as embolism beads (198.8 +/- 23.2 nm). Radioactive iodine-125 is labeled to Au@PDA to introduce the function of intra-irradiation. For the therapeutic effects of Au@PDA-I-125, Au particles sensitize the radiation to MHCC97H hepatoma cells and tumor-bearing mice. At the cellular level, after being treated with a relatively low-dose (5 Gy) -ray, Au-sensitized radiotherapy (RT) leads to an immediate increase of intracellular reactive oxygen species, accompanying with an increase of cell apoptosis. Due to the intra-irradiation, self-healing of RT-leaded DNA double-strand breakage is suppressed, inducing a further increase of cell apoptosis after RT treatment. Likewise, 3 cycles of sensitized RT leads to a valid control of tumor volume growth, but Au@PDA-I-125 has no harm or radioactive residual on or in the radiosensitive organs, including the thyroid, heart, lungs, liver, and spleen. Additionally, photons emitted from I-125 and high X-ray absorption of the Au element makes the beads suitable for single photon emission computed tomography/computed tomography (SPECT/CT) imaging. Therefore, as theranostic embolism beads, Au@PDA-I-125 can both enhance the therapeutic effects of external RT, and provide a real-time SPECT/CT monitoring of therapeutic time window.