A gene encoding an abscisic acid biosynthetic enzyme (LsNCED4) collocates with the high temperature germination locus Htg6.1 in lettuce (Lactuca sp.).
A gene encoding an abscisic acid biosynthetic enzyme (LsNCED4) collocates with the high temperature germination locus Htg6.1 in lettuce (Lactuca sp.).
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DOI：
10.1007/s00122-010-1425-3
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发表时间：
2011-01
影响因子：
5.4
通讯作者：
Bradford, Kent J.
中科院分区：
文献类型：
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作者：
Argyris, Jason;Truco, Maria Jose;Ochoa, Oswaldo;McHale, Leah;Dahal, Peetambar;Van Deynze, Allen;Michelmore, Richard W.;Bradford, Kent J.
热抑制，或种子发芽失败时，吸收在温暖的温度，可以是生菜（Lactuca sativa L.）生产的一个重大问题。通过提高生菜种子在高温下发芽的能力，可以提高立林的可靠性。在一个由苜蓿（L. sativa cv.）杂交而成的重组自交系群体中定位了编码发芽或休眠相关蛋白的基因。Salinas和L. serriola加入UC96US23。这揭示了几个候选基因位于包含与发芽所需温度和光照相关的数量性状位点（qtl）的基因组区域。特别是，萌发抑制剂脱落酸（ABA）生物合成途径中的温度调节基因LsNCED4定位到UC96US23高温萌发QTL （Htg6.1）的中心。在Htg6.1时，通过回交获得了3组与LsNCED4的UC96US23等位基因纯合的BC3S2姐妹近等基因系。盐碱地和标记辅助选择，然后是自交。含有UC96US23等位基因的NIL种子批次在LsNCED4位点的最高萌发温度比没有基因渗入的NIL种子批次提高了2 ~ 3℃。此外，LsNCED4在前者NIL中的表达量比后者低2 - 3倍。总之，这些数据强烈暗示LsNCED4是负责Htg6.1表型的候选基因，并表明在高吸吸温度下ABA生物合成减少是UC96US23种子萌发耐热性增加的主要因素。本文的在线版本（doi:10.1007/s00122-010-1425-3）包含补充材料，可供授权用户使用。
Thermoinhibition, or failure of seeds to germinate when imbibed at warm temperatures, can be a significant problem in lettuce (Lactuca sativa L.) production. The reliability of stand establishment would be improved by increasing the ability of lettuce seeds to germinate at high temperatures. Genes encoding germination- or dormancy-related proteins were mapped in a recombinant inbred line population derived from a cross between L. sativa cv. Salinas and L. serriola accession UC96US23. This revealed several candidate genes that are located in the genomic regions containing quantitative trait loci (QTLs) associated with temperature and light requirements for germination. In particular, LsNCED4, a temperature-regulated gene in the biosynthetic pathway for abscisic acid (ABA), a germination inhibitor, mapped to the center of a previously detected QTL for high temperature germination (Htg6.1) from UC96US23. Three sets of sister BC3S2 near-isogenic lines (NILs) that were homozygous for the UC96US23 allele of LsNCED4 at Htg6.1 were developed by backcrossing to cv. Salinas and marker-assisted selection followed by selfing. The maximum temperature for germination of NIL seed lots with the UC96US23 allele at LsNCED4 was increased by 2–3°C when compared with sister NIL seed lots lacking the introgression. In addition, the expression of LsNCED4 was two- to threefold lower in the former NIL lines as compared to expression in the latter. Together, these data strongly implicate LsNCED4 as the candidate gene responsible for the Htg6.1 phenotype and indicate that decreased ABA biosynthesis at high imbibition temperatures is a major factor responsible for the increased germination thermotolerance of UC96US23 seeds. The online version of this article (doi:10.1007/s00122-010-1425-3) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Grube, RC;Wintermantel, WM;Ryder, EJ
通讯作者：
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影响因子：
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影响因子：
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