Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration.
Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration.
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DOI：
10.3791/55535
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发表时间：
2017-05
期刊：
影响因子：
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通讯作者：
Menghon Cheah;J. Fawcett;Melissa R. Andrews
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文献类型：
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作者：
Menghon Cheah;J. Fawcett;Melissa R. Andrews
神经系统损伤后实现轴突再生是一项具有挑战性的任务。由于中枢神经系统（CNS）的不同部分在解剖学上存在差异，因此确定一个合适的模型用于轴突再生的研究是很重要的。通过使用合适的模型，我们可以根据损伤的严重程度、感兴趣的神经元细胞类型和评估再生所需的脊髓束来制定特定的治疗方案。在感觉通路中，DRG神经元负责将感觉信息从外周传递到中枢神经系统。我们在此提出了一种方案，使用带有病毒载体的DRG注射和成年大鼠下颈脊髓并发背根挤压损伤作为模型来研究感觉轴突再生。通过使用对照病毒AAV5-GFP，我们证明了直接注射DRG在转导DRG神经元和追踪感觉轴突进入脊髓中的有效性。我们还展示了前爪背根挤压损伤作为评估轴突再生的损伤模型的有效性。尽管进行这种侵入性手术需要专门的培训，但该方案是灵活的，潜在用户可以修改许多部件以适应他们的实验要求。重要的是，它可以为那些寻找合适的动物模型进行研究的人提供基础。我们相信这篇文章将帮助新用户以一种非常高效和有效的方式学习这个过程。
Achieving axon regeneration after nervous system injury is a challenging task. As different parts of the central nervous system (CNS) differ from each other anatomically, it is important to identify an appropriate model to use for the study of axon regeneration. By using a suitable model, we can formulate a specific treatment based on the severity of injury, the neuronal cell type of interest, and the desired spinal tract for assessing regeneration. Within the sensory pathway, DRG neurons are responsible for relaying sensory information from the periphery to the CNS. We present here a protocol that uses a DRG injection with a viral vector and a concurrent dorsal root crush injury in the lower cervical spinal cord of an adult rat as a model to study sensory axon regeneration. As demonstrated using a control virus, AAV5-GFP, we show the effectiveness of a direct DRG injection in transducing DRG neurons and tracing sensory axons into the spinal cord. We also show the effectiveness of the dorsal root crush injury in denervating the forepaw as an injury model for evaluating axon regeneration. Despite the requirement for specialized training to perform this invasive surgical procedure, the protocol is flexible, and potential users can modify many parts to accommodate their experimental requirements. Importantly, it can serve as a foundation for those in search of a suitable animal model for their studies. We believe that this article will help new users to learn the procedure in a very efficient and effective manner.