Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges.
Metagenomic discovery of polybrominated diphenyl ether biosynthesis by marine sponges.
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DOI：
10.1038/nchembio.2330
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发表时间：
2017-05
影响因子：
14.8
通讯作者：
Moore BS
中科院分区：
文献类型：
--
作者：
Agarwal V;Blanton JM;Podell S;Taton A;Schorn MA;Busch J;Lin Z;Schmidt EW;Jensen PR;Paul VJ;Biggs JS;Golden JW;Allen EE;Moore BS
自然产生的多溴联苯醚（PBDEs）遍布海洋环境，其结构类似于有毒的人造溴化阻燃剂。多溴二苯醚在海洋动物体内会发生生物累积，并可能转移到人类食物链中。然而，在多溴二苯醚最丰富的来源之一-异海绵科海绵中产生多溴二苯醚的生物学基础仍未查明。在这里，我们报告发现PBDE生物合成基因簇海绵微生物组相关的蓝藻内共生体采用公正的宏基因组挖掘方法。通过在异源蓝藻宿主中表达PBDE生物合成基因，我们将天然产生的PBDE的结构多样性与多个海绵holobiont中PBDE生物合成基因簇的修饰相关联。我们的研究结果建立了遗传和分子基础的生产多溴联苯醚在这些分子的最丰富的自然来源之一，进一步设置为一个宏基因组为基础的清单在海洋环境中的其他多溴联苯醚来源。
Naturally produced polybrominated diphenyl ethers (PBDEs) pervade the marine environment and structurally resemble toxic man-made brominated flame retardants. PBDEs bioaccumulate in marine animals and are likely transferred to the human food chain. However, the biogenic basis for PBDE production in one of their most prolific sources, marine sponges of the order Dysideidae, remains unidentified. Here, we report the discovery of PBDE biosynthetic gene clusters within sponge microbiome-associated cyanobacterial endosymbionts by employing an unbiased metagenome mining approach. By expression of PBDE biosynthetic genes in heterologous cyanobacterial hosts, we correlate the structural diversity of naturally produced PBDEs to modifications within PBDE biosynthetic gene clusters in multiple sponge holobionts. Our results establish the genetic and molecular foundation for the production of PBDEs in one of the most abundant natural sources of these molecules, further setting the stage for a metagenomic-based inventory of other PBDE sources in the marine environment.