Electron cryo-tomography captures macromolecular complexes in native environments
Electron cryo-tomography captures macromolecular complexes in native environments
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DOI：
10.1016/j.sbi.2017.08.005
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发表时间：
2017-10-01
影响因子：
6.8
通讯作者：
Grunewald, Kay
中科院分区：
文献类型：
--
作者：
Baker, Lindsay A.;Grange, Michael;Grunewald, Kay
透射电子显微镜在细胞生物学中有着悠久的历史。固定和染色样品已用于细胞成像超过50年，但遭受样品制备诱导伪影。电子冷冻断层扫描（cryoET）使用冷冻水合样品，而不进行化学修饰，以确定大分子复合物在其天然环境中的结构。电子显微镜和相关技术的最新发展极大地扩展了我们可视化细胞特征和原位确定大分子复合物结构的能力。这篇综述强调了技术进步和这些进步所带来的生物学新领域。我们讨论了低温低温技术在纳米或亚纳米尺度上揭示新的重要生物学信息的潜力，以及进一步工作的方向。
Transmission electron microscopy has a long history in cellular biology. Fixed and stained samples have been used for cellular imaging for over 50 years, but suffer from sample preparation induced artifacts. Electron cryo-tomography (cryoET) instead uses frozen-hydrated samples, without chemical modification, to determine the structure of macromolecular complexes in their native environment. Recent developments in electron microscopes and associated technologies have greatly expanded our ability to visualize cellular features and determine the structures of macromolecular complexes in situ. This review highlights the technological improvements and the new areas of biology these advances have made accessible. We discuss the potential of cryoET to reveal novel and significant biological information on the nanometer or subnanometer scale, and directions for further work.