Exercise improves import of 8-oxoguanine DNA glycosylase into the mitochondrial matrix of skeletal muscle and enhances the relative activity
Exercise improves import of 8-oxoguanine DNA glycosylase into the mitochondrial matrix of skeletal muscle and enhances the relative activity
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DOI：
10.1016/j.freeradbiomed.2008.10.022
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发表时间：
2009-01-15
影响因子：
7.4
通讯作者：
Goto, Sataro
中科院分区：
文献类型：
--
作者：
Radak, Zsolt;Atalay, Mustafa;Goto, Sataro
运动已被证明可以改变骨骼肌中DNA损伤修复酶8-氧代鸟嘌呤-DNA糖基化酶（OGG 1）的水平/活性。我们研究了常规体育训练（8周游泳）和停训（8周训练后8周测试）对细胞核和线粒体中OGG 1活性的影响，以及其靶向骨骼肌中线粒体基质的影响。无论是运动训练，也不停止训练改变反应性物种的整体水平，然而，在训练组的羰基化蛋白质的线粒体水平下降的电子自旋共振和生化方法进行评估。重要的是，核OGG 1活性增加日常运动训练，而停止训练逆转了训练的上调作用。有趣的是，训练降低了外膜相关的线粒体OGG 1水平，而停止训练则逆转了这种作用。这些结果表明，运动训练改善OGG 1输入到线粒体基质，从而增加OGG 1介导的氧化鸟嘌呤碱基的修复。综上所述，我们的数据表明，身体不活动可能会损害OGG 1的线粒体靶向作用;然而，运动训练增加了OGG 1在细胞核中的水平/活性以及OGG 1在线粒体区室中的比活性，从而增强了氧化的细胞核和线粒体DNA碱基的修复。(C)2008年爱思唯尔公司All rights reserved.
Exercise has been shown to modify the level/activity of the DNA damage repair enzyme 8-oxoguanine-DNA glycosylase (OGG1) in skeletal muscle. We have studied the impact of regular physical training (8 weeks of swimming) and detraining (8 weeks of test after an 8-week training session) on the activity of OGG1 in the nucleus and mitochondria as well as its targeting to the mitochondrial matrix in skeletal muscle. Neither exercise training nor detraining altered the overall levels of reactive species; however, mitochondrial levels of carbonylated proteins were decreased in the trained group as assessed by electron spin resonance and biochemical approaches. Importantly, nuclear OGG1 activity was increased by daily exercise training, whereas detraining reversed the up-regulating effect of training. interestingly, training decreased the outermembrane-associated mitochondrial OGG1 levels, whereas detraining reversed this effect. These results suggest that exercise training improves OGG1 import into the mitochondrial matrix, thereby increasing OGG1-mediated repair of oxidized guanine bases. Taken together, Our data suggest that physical inactivity could impair the mitochondrial targeting of OGG1; however, exercise training increases OGG1 levels/activity in the nucleus and specific activity of OGG1 in mitochondrial compartments, thereby augmenting the repair of oxidized nuclear and mitochondrial DNA bases. (C) 2008 Elsevier Inc. All rights reserved.