Life cycle of an endogenous retrovirus, ZAM in Drosophila melanogaster
Life cycle of an endogenous retrovirus, ZAM in Drosophila melanogaster
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DOI：
10.1128/jvi.74.22.10658-10669.2000
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发表时间：
2000-11-01
影响因子：
5.4
通讯作者：
Vaury, C
中科院分区：
文献类型：
--
作者：
Leblanc, P;Desset, S;Vaury, C
ZAM是吉普赛反转录转座子家族中一个含有env的成员，它代表了一种可能的无脊椎动物逆转录病毒。在这篇文章中，我们追踪ZAM的动员，以获得关于逆转录元件可能采取的潜在路径的信息，以到达其宿主的胚系。整体组织的原位杂交和针对ZAM Gag和Env蛋白的抗体的免疫细胞化学分析表明，组装ZAM病毒颗粒所需的所有成分，即ZAM全长RNA和Gag和Env多肽，在卵母细胞周围的一小部分滤泡细胞中共表达。通过电子显微镜，我们已经证明ZAM病毒颗粒确实在这个体细胞谱系中被检测到，它们离开并进入紧密相对的卵母细胞。我们的数据证明卵黄发生过程中的囊泡运输和卵黄颗粒在ZAM转移到卵母细胞中起着重要的作用。我们的数据支持这样一种可能性，即卵黄蛋白原转移到卵母细胞可能会帮助反转录元件在不需要其包膜产物的情况下传递到生殖系。
ZAM is an env-containing member of the gypsy family of retrotransposons that represents a possible retrovirus of invertebrates. In this paper, we traced ZAM mobilization to get information about a potential path a retroelement may take to reach the germ line of its host. In situ hybridization on whole-mount tissues and immunocytochemistry analyses with antibodies raised against ZAM Gag and Env proteins have shown that all components necessary to assemble ZAM viral particles, i.e., ZAM full-length RNAs and Gag and Env polypeptides, are coexpressed in a small set of follicle cells surrounding the oocyte. By electron microscopy, we have shown that ZAM viral particles are indeed detected in this somatic lineage of cells, which they leave and enter the closely apposed oocyte. Our data provide evidence that the vesicular traffic and yolk granules in the process of vitellogenesis play an important role in ZAM transfer to the oocyte. Our data support the possibility that vitellogenin transfer to the oocyte may help a retroelement pass to the germ line with no need of its envelope product.