Metabolomics Insights into the Modulatory Effects of Long-Term Low Calorie Intake in Mice
Metabolomics Insights into the Modulatory Effects of Long-Term Low Calorie Intake in Mice
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代谢组学洞察小鼠长期低热量摄入的调节作用
DOI：
10.1021/acs.jproteome.6b00336
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发表时间：
2016
影响因子：
4.4
通讯作者：
Tang Huiru
中科院分区：
文献类型：
--
作者：
Wu Junfang;Yang Liu;Li Shoufeng;Huang Ping;Liu Yong;Wang Yulan;Tang Huiru
越来越多的证据表明，在没有营养不良的情况下限制卡路里可以延长寿命，并推迟与年龄有关的疾病的发生。确定与不同饮食习惯相关的生化扰动将为新陈代谢和长寿之间的关联提供有价值的见解。为了揭示长期饮食干预对代谢紊乱的影响，我们使用基于NMR的代谢组学研究了小鼠一生中交互式高/低脂饮食结合/不降低热量摄入引起的血清和尿液代谢变化。我们发现，高热量饮食制度扰乱脂质代谢，抑制糖酵解和TCA循环，刺激氧化应激，促进核苷酸代谢和代谢产物生成，并扰乱肠道微生物-宿主相互作用。这种变化可以通过长期低热量摄入来改变。最重要的是，我们发现，无论饮食制度的热量摄入指数对代谢紊乱发挥主导作用。我们的调查提供了一个整体的观点，长期的饮食干预，这是重要的检测生理变化和饮食对哺乳动物代谢的影响代谢的影响。
There is increasing evidence that calorie restriction without malnutrition can extend longevity and delay the onset of age-associated disorders. Identifying the biochemical perturbations associated with different dietary habits would provide valuable insights into associations between metabolism and longevity. To reveal the effects of long-term dietary interventions on metabolic perturbations, we investigated serum and urinary metabolic changes induced by interactive high/low fat diet in combination with/without reduced caloric intake over a life span in mice using NMR-based metabonomics. We found that the high calorie dietary regime disturbed lipid metabolism, suppressed glycolysis and TCA cycles, stimulated oxidative stress, promoted nucleotide metabolism and gluconeogenesis, and perturbed gut microbiota–host interactions. Such changes could be modified by long-term low calorie intake. Most importantly, we found that the calorie intake index exerts a dominant effect on metabolic perturbations irrespective of dietary regime. Our investigation provides a holistic view of the metabolic impact of long-term dietary interventions, which are important for detecting physiological changes and dietary effects on mammalian metabolism.