Dual substrates biodegradation kinetics of m-cresol and pyridine by Lysinibacillus cresolivorans.
Dual substrates biodegradation kinetics of m-cresol and pyridine by Lysinibacillus cresolivorans.
复制标题
DOI：
10.1016/j.jhazmat.2010.11.118
复制
发表时间：
2011-02
影响因子：
13.6
通讯作者：
Haiyan Yao;Yuan Ren;Xiuqiong Deng;Chao-hai Wei
中科院分区：
文献类型：
--
作者：
Haiyan Yao;Yuan Ren;Xiuqiong Deng;Chao-hai Wei
酚类化合物和氮杂环类化合物在实际废水中共存，尤其是在石化废水和焦化废水中。以嗜甲苯酚赖氨酸芽孢杆菌（Lysinibacillus cresolivorans）为菌株，研究了间甲酚和吡啶作为单一和双重底物时的底物相互作用。在间甲酚初始浓度为0 ~ 1200 mg/L、吡啶初始浓度为0 ~ 150 mg/L的条件下，考察了菌体生长和底物降解动力学。单底物反应动力学可以用Halfenic动力学模型很好地描述。间甲酚对细胞生长的单底物参数值为μmax=0.89h-1，Ks=426.25mg/L，Ki=51.26mg/L;对吡啶底物，μmax=0.0925h-1，Ks=60.28mg/L，Ki=16.17mg/L。当细胞在混合基质上生长时，观察到基质的抑制作用。相互作用参数Im，p（0.76）大于Im，p（0.11），表明间甲酚对吡啶利用的抑制作用远大于吡啶对间甲酚生物降解的抑制作用。研究表明，L.在降解间甲酚和吡啶的混合底物中，
Phenols and N-heterocyclic compounds are found to co-exist in actual wastewater, especially in petrochemical and coking wastewater. Lysinibacillus cresolivorans, a bacterium capable of phenol-biodegradation was used to study the substrate interactions of m-cresol and pyridine as single and dual substrates. The cell growth and substrate biodegradation kinetics were also investigated with initial m-cresol concentrations varying from 0 to 1200mg/L and pyridine concentrations varying from 0 to 150mg/L. The single substrate kinetics was well described by the Haldane kinetic models. The single-substrate parameter values of m-cresol on cell growth were μmax=0.89h−1, Ks=426.25mg/L, Ki=51.26mg/L and μmax=0.0925h−1, Ks=60.28mg/L, Ki=16.17mg/L for cell growth on pyridine. Inhibitory effects of substrates were observed when cells were grown on the mixed substrates. The interaction parameter Im,p(0.76) was greater than Im,p(0.11), which indicated that m-cresol inhibited the utilization of pyridine much more than pyridine inhibited the biodegradation of m-cresol. The study showed a good potential of L. cresolivorans in degrading mixed substrates of m-cresol and pyridine.