Nonrenewal of Neurons in the Cerebral Neocortex of Adult Macaque Monkeys
Nonrenewal of Neurons in the Cerebral Neocortex of Adult Macaque Monkeys
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DOI：
10.1523/jneurosci.23-03-00937.2003
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发表时间：
2003-02
期刊：
影响因子：
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通讯作者：
Daisuke Koketsu;A. Mikami;Y. Miyamoto;T. Hisatsune
中科院分区：
文献类型：
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作者：
Daisuke Koketsu;A. Mikami;Y. Miyamoto;T. Hisatsune
发育期后，新皮质神经元在数量上变得稳定并且通常不可再生的概念受到了成年猕猴大脑皮质联合区持续神经发生的报告的挑战。因此，我们重新研究了这个问题，在两个不同的猕猴物种使用胸苷类似物溴脱氧尿苷（BrdU）作为细胞分裂过程中DNA复制的指标。我们发现了几个BrdU+/NeuN+（神经元核）双标记细胞，但皮质神经元，区分其大小和细胞学和免疫组化特性，没有BrdU阳性。我们详细检查了额叶皮层，据说那里每天增加的神经元最多，但没有看到迁移流或任何新神经元增加的迹象。因此，我们的结论是，在正常情况下，成年灵长类动物的皮质神经元，类似于其他哺乳动物物种，既不补充，也不更新。
The concept that, after developmental periods, neocortical neurons become numerically stable and are normally nonrenewable has been challenged by a report of continuous neurogenesis in the association areas of the cerebral cortex in the adult Macaque monkey. Therefore, we have reexamined this issue in two different Macaque species using the thymidine analog bromodeoxyuridine (BrdU) as an indicator of DNA replication during cell division. We found several BrdU+/NeuN+ (neuronal nuclei) double-labeled cells, but cortical neurons, distinguished readily by their size and cytological and immunohistochemical properties, were not BrdU positive. We examined in detail the frontal cortex, where it is claimed that the largest daily addition of neurons has been made, but did not see migratory streams or any sign of addition of new neurons. Thus, we concluded that, in the normal condition, cortical neurons of adult primates, similar to other mammalian species, are neither supplemented nor renewable.