High genetic diversity vs. low genetic differentiation in Nouelia insignis (Asteraceae), a narrowly distributed and endemic species in China, revealed by ISSR fingerprinting
High genetic diversity vs. low genetic differentiation in Nouelia insignis (Asteraceae), a narrowly distributed and endemic species in China, revealed by ISSR fingerprinting
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DOI：
10.1093/aob/mcl129
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发表时间：
2006-09-01
期刊：
影响因子：
4.2
通讯作者：
Gong, Xun
中科院分区：
文献类型：
--
作者：
Luan, Shanshan;Chiang, Tzen-Yuh;Gong, Xun
中心点背景和目的Nouelia insignis Franch.，菊科单型属，中国云南、四川两省特有濒危种。大多数人都受到严重威胁。有些甚至濒临灭绝。方法采用基于简单重复序列多态性的DNA指纹图谱技术，分析了两个流域的黄草种群的遗传多样species.center分化。结果黄草种群水平的遗传多样性较高，多态位点百分率P = 65.05%，总遗传多样性H-t = 0.2248。areas.center通过对总基因多样性的划分，估算出居群间的遗传分化系数G（st）为0.2529;而金沙和南盘江流域的群体间遗传分化则较低（G（st）= 0.0702）。中心点结论根据DNA指纹图谱的遗传分析，最近的生境破碎化可能没有导致稀有物种的遗传分化或遗传多样性的丧失。指纹多态性的空间分配提供了跨越地理障碍的历史迁移足迹。在这项研究中发现的高度多样性为保护和恢复工作提供了希望，以拯救濒危物种免于灭绝。
center dot Background and Aims Nouelia insignis Franch., a monotypic genus of the Asteraceae, is an endangered species endemic in Yunnan and Sichuan Provinces of China. Most of the populations are seriously threatened. Some of them are even at the brink of extinction. In this study, the genetic diversity and differentiation between populations of this species were examined in two drainage areas.center dot Methods DNA fingerprinting based on inter-simple sequence repeat polymorphisms was employed to detect the genetic variation and population structure in the species.center dot Key Results Genetic diversity at species level was high with P = 65.05 % (percentage of polymorphic loci) and H-t = 0.2248 (total genetic diversity). The coefficient of genetic differentiation among populations, G(st), which was estimated by partitioning the total gene diversity, was 0.2529; whereas, the genetic differentiation between populations in the Jinsha and Nanpan drainage areas was unexpectedly low (G(st) = 0.0702).center dot Conclusions Based on the genetic analyses of the DNA fingerprinting, recent habitat fragmentation may not have led to genetic differentiation or the loss of genetic diversity in the rare species. Spatial apportionment of fingerprinting polymorphisms provides a footprint of historical migration across geographical barriers. The high diversity detected in this study holds promise for conservation and restoration efforts to save the endangered species from extinction.