The claudin-like Megatrachea is essential in septate junctions for the epithelial barrier function in Drosophila
The claudin-like Megatrachea is essential in septate junctions for the epithelial barrier function in Drosophila
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DOI：
10.1016/s1534-5807(03)00275-2
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发表时间：
2003-10-01
影响因子：
11.8
通讯作者：
Schuh, R
中科院分区：
文献类型：
--
作者：
Behr, M;Riedel, D;Schuh, R
脊椎动物紧密连接蛋白是紧密连接的组成部分，在上皮细胞中起细胞旁扩散屏障的作用。我们确定Megalehea（兆），果蝇跨膜蛋白同源的claudins，并表明它的行为在隔膜连接，相应的结构的无脊椎动物。我们的分析表明，Mega具有与claudins相似的跨上皮屏障功能。此外，Mega是正常气管细胞形态发生所必需的，但不是顶基极性或上皮完整性所必需的。此外，我们提出的证据表明，兆是必不可少的分隔连接蛋白复合物Coracle/Neurexin的本地化。结果表明，类claudin蛋白在脊椎动物和果蝇之间是功能保守的。
Vertebrate claudin proteins are integral components of tight junctions, which function as paracellular diffusion barriers in epithelia. We identified Megatrachea (Mega), a Drosophila transmembrane protein homologous to claudins, and show that it acts in septate junctions, the corresponding structure of invertebrates. Our analysis revealed that Mega has transepithelial barrier function similar to the claudins. Also, Mega is necessary for normal tracheal cell morphogenesis but not for apicobasal polarity or epithelial integrity. In addition, we present evidence that Mega is essential for localization of the septate junction protein complex Coracle/Neurexin. The results indicate that claudin-like proteins are functionally conserved between vertebrates and Drosophila.