High-mobility group proteins 14 and 17 maintain the timing of early embryonic development in the mouse
High-mobility group proteins 14 and 17 maintain the timing of early embryonic development in the mouse
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DOI：
10.1006/dbio.2000.9942
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Clarke, HJ
中科院分区：
文献类型：
--
作者：
Mohamed, OA;Bustin, M;Clarke, HJ
高迁移率族（HMG）蛋白14和17是与核小体结合并增强转录的丰富的染色体蛋白。我们报告，这两种mRNA的物种和蛋白质都存在于整个卵子发生和植入前发育的小鼠。当将针对每种mRNA的反义寡核苷酸注射到单细胞胚胎中时，蛋白质在两细胞和四细胞阶段被耗尽，并在八细胞阶段重新积累。用靶向HMG-14和HMG-17的反义寡核苷酸注射的单细胞胚胎裂解至两细胞阶段。然而，与对照注射的胚胎相比，随后的卵裂被延迟。然而，这些胚胎最终达到囊胚阶段。类似地，将对应于HMG-14和HMG-17的共同核小体结合结构域的肽注射到两细胞胚胎的细胞核中可以延迟向四细胞阶段的进展。此外，与注射对照相比，在反义注射的胚胎中RNA和蛋白质合成都短暂减少。这些结果确定了HMG-14和HMG-17作为小鼠卵母细胞和胚胎染色质的组成成分，并建立了胚胎染色质的结构和胚胎发育的正常进程之间的联系。(C)北京：科学出版社.
The high-mobility group (HMG) proteins 14 and 17 are abundant chromosomal proteins that bind to nucleosomes and enhance transcription. We report that both mRNA species and both proteins are present throughout oogenesis and preimplantation development of the mouse. When antisense oligonudeotides targeting each mRNA species are injected into one-cell embryos, the proteins become depleted at the two- and four-cell stages and reaccumulate at the eight-cell stage. One-cell embryos injected with antisense oligonucleotides targeting both HMG-14 and HMG-17 cleave to the two-cell stage. Subsequent cleavages, however, are delayed compared with control-injected embryos. Nevertheless, these embryos ultimately reach the blastocyst stage. Similarly, injection into the nuclei of two-cell embryos of a peptide corresponding to the common nucleosome-binding domain of HMG-14 and HMG-17 delays progression to the four-cell stage. Furthermore, both RNA and protein synthesis is transiently reduced in antisense-injected embryos compared with injected controls. These results identify HMG-14 and HMG-17 as constitutive components of mouse oocyte and embryonic chromatin and establish a link between the structure of embryonic chromatin and the normal progression of embryonic development. (C) 2001 Academic Press.