Comparison of composition-gradient sedimentation equilibrium and composition-gradient static light scattering as techniques for quantitative characterization of biomolecular interactions: A case study
Comparison of composition-gradient sedimentation equilibrium and composition-gradient static light scattering as techniques for quantitative characterization of biomolecular interactions: A case study
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DOI：
10.1016/j.ab.2019.06.010
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发表时间：
2019-10-15
影响因子：
2.9
通讯作者：
Minton，Allen P.
中科院分区：
文献类型：
--
作者：
Arisaka，Fumio;Niimura，Youichi;Minton，Allen P.
通过独立进行的组成-梯度沉降平衡和组成-梯度静态光散射的测量，表征了NADH氧化酶和过氧化还蛋白的平衡异结合。这两个实验的结果被一个模型定量地解释，根据该模型，在这些实验的条件下，NADH氧化酶的二聚体与过氧化还蛋白的十聚体形成1：1的平衡络合物。由这两种方法得到的两种蛋白质的最佳异构化平衡常数在两种方法的估计不确定度内是相同的。讨论了每种方法的相对优点。
The equilibrium hetero-association of NADH oxidase and peroxiredoxin was characterized by means of independently conducted measurements of composition-gradient sedimentation equilibrium and composition-gradient static light scattering. Results obtained from both experiments were quantitatively accounted for by a model according to which a dimer of NADH oxidase forms a 1:1 equilibrium complex with a decamer of peroxiredoxin under the conditions of these experiments. The best-fit equilibrium constants for heteroassociation of the two proteins obtained from the two measurements were found to be identical to well within the uncertainty of estimate of each of the two methods. The relative virtues of each of the methods are discussed.