mRNA up-regulation of MHC II and pivotal pro-inflammatory genes in normal brain aging
mRNA up-regulation of MHC II and pivotal pro-inflammatory genes in normal brain aging
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DOI：
10.1016/j.neurobiolaging.2005.03.013
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发表时间：
2006-05-01
影响因子：
4.2
通讯作者：
Maier, SF
中科院分区：
文献类型：
--
作者：
Frank, MG;Barrientos, RA;Maier, SF
在正常脑老化中，CNS驻留巨噬细胞表现出主要组织相容性复合物（MHC）11表达的增加。然而，这一观察结果的转录基础尚未澄清，也没有关键促炎基因的年龄相关变化的特点。在年龄较大（24个月）和较年轻（3个月）的雄性F344 xBN F1大鼠中测量了MHC 11、MHC 11辅助分子和几种促炎介质中的MHC 11相关mRNA变化。利用真实的时间RT-PCR来测量鲤鱼中的稳态mRNA水平。与年轻动物相比，老年动物表现出MHC II、CD 86、CIITA和IFN-γ的mRNA水平增加。此外，IL-10和CD 200 mRNA，下调巨噬细胞活化的分子，在老年动物中减少。目前的结果表明，正常的脑老化的特点是在中枢神经系统的促炎微环境的转变。(c)2005年爱思唯尔公司All rights reserved.
In normal brain aging, CNS resident macrophages exhibit increased expression of major histocompatibility complex (MHC) 11 expression. However, the transcriptional basis for this observation has not been clarified nor have age-related alterations in pivotal pro-inflammatory genes been characterized. Age-related mRNA alterations in MHC 11, MHC 11 accessory molecules and several pro-inflammatory mediators were measured in older (24 months) and younger (3 months) male F344xBN F1 rats. Real time RT-PCR was utilized to measure steady state mRNA levels in hippocarnpus. Older as compared to younger animals exhibited increased mRNA levels of MHC II, CD86, CIITA and IFN-gamma. Furthermore, IL-10 and CD200 mRNA, molecules that down-regulate macrophage activation, was decreased in older animals. The present results indicate that normal brain aging is characterized by a shift towards a pro-inflammatory microenvironment in the CNS. (c) 2005 Elsevier Inc. All rights reserved.