Characterization of phenanthrene degradation by strain Polyporus sp S133
Characterization of phenanthrene degradation by strain Polyporus sp S133
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DOI：
10.1016/s1001-0742(09)60085-1
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发表时间：
2010-01-01
影响因子：
6.9
通讯作者：
Tachibana, Sanro
中科院分区：
文献类型：
--
作者：
Hadibarata, Tony;Tachibana, Sanro
利用多孔菌S133在无机盐液体培养基中降解多环芳烃菲。在120 r/min搅拌条件下培养30 d，多孔菌S133的降解率最高，为92%，而非搅拌条件下的降解率为44%。此外，降解的影响，通过添加表面活性剂。吐温80是多孔菌S133降解菲的最佳表面活性剂。随着吐温80添加量的增加，降解率增加。搅拌培养中的速率约为非搅拌培养中的2倍。通过鉴定代谢产物9，10-菲醌、2，2 '-联苯二甲酸、邻苯二甲酸和原儿茶酸，确定了降解机制。在培养过程中检测到多孔菌S133产生的几种酶（锰过氧化物酶、木质素过氧化物酶、漆酶、1，2-双加氧酶和2，3-双加氧酶）。培养20天后，1，2-双加氧酶活性最高（187.4 U/L）。
Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate (92%) was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9, 10-phenanthrenequinone, 2,2'-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes (manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase) produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase (187.4 U/L) after 20 days of culture.