TRPV1 activation is involved in the cardioprotection of remote limb ischemic postconditioning in ischemia-reperfusion injury rats
TRPV1 activation is involved in the cardioprotection of remote limb ischemic postconditioning in ischemia-reperfusion injury rats
复制标题
TRPV1激活参与缺血再灌注损伤大鼠远端肢体缺血后处理的心脏保护作用
DOI：
10.1016/j.bbrc.2015.06.054
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发表时间：
2015-08-07
影响因子：
3.1
通讯作者：
Lee, Chongyou
中科院分区：
文献类型：
--
作者：
Gao, Yuanfeng;Song, Junxian;Lee, Chongyou
肢体远端缺血后处理（Limb remote ischemic postconditioning，RIPostC）是一种安全、有效的心肌保护措施。但是，是什么连接了远端器官损伤和心脏保护作用仍有待阐明。本研究旨在探讨TRPV 1是否介导了从远隔器官到心脏的心脏保护作用，以及CGRP和SP在这一过程中的作用。我们发现，RIPostC有效地改善心肌缺血/再灌注损伤，限制梗死面积，降低CK和cTnI释放，改善心功能。此外，这些心脏保护作用可以通过在再灌注前注射相应的拮抗剂（CGRP 8 -37和RP-67580）抑制CGRP或SP受体而显著消除。此外，RIPostC还可显著增加血浆和心脏中CGRP和SP的含量，以及DRG中CGRP和SP的含量和mRNA表达。TRPV 1受体拮抗剂辣椒平可明显抑制血浆和心脏CGRP和SP水平的升高。提示肢体缺血后处理可减轻大鼠心肌缺血/再灌注损伤，其机制可能与TRPV 1上调CGRP和SP的表达，进而作用于心肌组织中相应的受体有关。(C)2015 Elsevier Inc. All rights reserved.
Limb remote ischemic postconditioning (RIPostC) has been proved to be a safe and effective measurement of cardioprotection against ischemia-reperfusion injury. But what bridges the remote organ insult and the cardioprotective effect in heart remains to be elucidated. This study aimed to found that whether TRPV1 may mediate the cardioprotective effect from remote organ to heart and the role of CGRP and SP in this process. We found that RIPostC effectively ameliorated cardiac ischemia/reperfusion injury in terms of limiting infarct size, lowering CK and cTnI release and improving cardiac function. In addition, these cardioprotective effects could be significantly abolished by inhibition of either CGRP or SP receptors with corresponding antagonists (CGRP8-37 for CGRP and RP-67580 for SP) injected before reperfusion. Besides, RIPostC resulted in significantly increase in the levels of CGRP and SP in plasma and hearts, as well as the levels and mRNA expression of CGRP and SP in DRG. The increase in CGRP and SP levels in plasma and hearts were markedly inhibited by TRPV1 receptor antagonist capsazepine. These findings indicate that limb remote ischemic postconditioning could attenuate cardiac ischemia/reperfusion injury in rats, and the cardioprotective mechanism is via TRPV1-mediated upregulation of CGRP and SP, which could subsequently act on their corresponding receptors in heart tissue. (C) 2015 Elsevier Inc. All rights reserved.