ON ROLE OF RESPONSE OF CELL MEMBRANE IN DETERMINING VIRUS VIRULENCE - CONTRASTING EFFECTS OF PARAINFLUENZA VIRUS SV5 IN 2 CELL TYPES
ON ROLE OF RESPONSE OF CELL MEMBRANE IN DETERMINING VIRUS VIRULENCE - CONTRASTING EFFECTS OF PARAINFLUENZA VIRUS SV5 IN 2 CELL TYPES
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DOI：
10.1084/jem.124.3.501
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发表时间：
1966-01-01
影响因子：
15.3
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
--
作者：
HOLMES, KV;CHOPPIN, PW
猴粘病毒SV5在小仓鼠肾(BHK21-F)细胞的连续系中繁殖，导致广泛的细胞融合，随后细胞死亡。接种15pfu/细胞后，潜伏期为7h，倍增时间约为60min，产量为7pfu/细胞。巨细胞的形成在感染后约6小时开始，14至18小时迅速发展到形成单一合胞体，24至36小时解体。SV5在原代恒河猴肾细胞中长时间繁殖，病毒产量高，细胞病变作用小。在无病毒增殖的情况下，SV5在1小时内可诱导BHK21-F细胞融合，但对猴肾细胞无明显影响。延时显微摄影显示，感染细胞融合形成巨细胞，部分多核细胞分裂。在多核细胞的异常分裂过程中，由几个亲本核物质形成巨核。免疫荧光显示BHK21-F细胞和猴肾细胞中病毒抗原的胞质发育相似。在猴肾细胞中，细胞DNA、RNA和蛋白质的合成不会因SV5感染而停止，而在BHK21-F细胞中，这些大分子的合成直到感染后12至15小时发生广泛的细胞融合后才被抑制。持续感染的BHK21-F细胞和猴肾细胞分别连续传了11代和28代。结果表明，SV5是像猴肾细胞那样作为中度病毒，还是像在BHK21-F细胞那样作为强毒，取决于细胞膜对病毒的反应。
The simian myxovirus SV5 multiplies in a continuous line of baby hamster kidney (BHK21-F) cells causing extensive cell fusion, followed by cell death. After inoculation of 15 PFU/cell, the latent period was 7 hr, the doubling time approximately 60 min, and the yield 7 PFU per cell. Giant cell formation began about 6 hr after infection and rapidly progressed to the formation by 14 to 18 hr of a single syncytium which disintegrated by 24 to 36 hr. In contrast, SV5 multiplies in primary rhesus monkey kidney cells for long periods of time producing high yields of virus with little cytopathic effect.High multiplicities of SV5 induced cell fusion in BHK21-F cells within 1 hr in the absence of virus multiplication but had no visible effect on monkey kidney cells.Time-lapse photomicrography has demonstrated that giant cells form by fusion of infected cells, and that some polykaryocytes divide. During aberrant division of polykaryocytes giant nuclei are formed from the nuclear material of several parent nuclei.The cytoplasmic development of viral antigens as demonstrated by immunofluorescence is similar in BHK21-F and monkey kidney cells. Synthesis of cellular DNA, RNA, and protein in monkey kidney cells is not shut off by SV5-infection, and in BHK21-F cells synthesis of these macromolecules is not inhibited until after extensive cell fusion has occurred 12 to 15 hr after infection. Persistently infected BHK21-F and monkey kidney cells have been serially carried through 11 and 28 cell passages, respectively.The results suggest that whether SV5 acts as a moderate virus, as in monkey kidney cells, or a virulent virus, as in BHK21-F cells, depends on the response of the cell membrane to the virus.