An Abrupt Shift in the Day/Night Cycle Causes Desynchrony in the Mammalian Circadian Center
An Abrupt Shift in the Day/Night Cycle Causes Desynchrony in the Mammalian Circadian Center
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DOI：
10.1523/jneurosci.23-14-06141.2003
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发表时间：
2003-07
期刊：
影响因子：
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通讯作者：
M. Nagano;A. Adachi;K. Nakahama;Toru Nakamura;M. Tamada;E. Meyer-Bernstein;A. Sehgal;Y. Shigeyoshi
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文献类型：
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作者：
M. Nagano;A. Adachi;K. Nakahama;Toru Nakamura;M. Tamada;E. Meyer-Bernstein;A. Sehgal;Y. Shigeyoshi
视交叉上核(SCN)是哺乳动物昼夜节律起搏器的神经解剖部位。在这里，我们证明了光/暗(LD)周期的突然变化扰乱了大鼠SCN中昼夜节律成分的同步振荡。周期基因PER1和PER2以及隐花色素基因Cry1的RNA周期在SCN的腹外侧光感受区移位较快，但在背内侧移位相对较慢。在去同步期，动物夜间休息时间增加，夜间休息时间与背内侧SCN中PER1基因表达呈负相关。分子再同步需要∼在延迟10小时后6天，在LD周期提前6小时后需要9∼13天，并伴随着正常静息-活动模式的重新出现。在LD周期移动后，SCN内内源振荡器的这种解离和缓慢的再同步表明了构成时差的生理症状的机制。
The suprachiasmatic nucleus (SCN) is the neuroanatomical locus of the mammalian circadian pacemaker. Here we demonstrate that an abrupt shift in the light/dark (LD) cycle disrupts the synchronous oscillation of circadian components in the rat SCN. The phases of the RNA cycles of the period genes Per1 and Per2 and the cryptochrome gene Cry1 shifted rapidly in the ventrolateral, photoreceptive region of the SCN, but were relatively slow to shift in the dorsomedial region. During the period of desynchrony, the animals displayed increased nighttime rest, the timing of which was inversely correlated with the expression of Per1 mRNA in the dorsomedial SCN. Molecular resynchrony required ∼6 d after a 10 hr delay and 9∼13 d after a 6 hr advance of the LD cycle and was accompanied by the reemergence of normal rest–activity patterns. This dissociation and slow resynchronization of endogenous oscillators within the SCN after an LD cycle shift suggests a mechanism for the physiological symptoms that constitute jet lag.