ANTIGENIC STRUCTURE OF POLIOVIRUS IN INACTIVATED VACCINES
ANTIGENIC STRUCTURE OF POLIOVIRUS IN INACTIVATED VACCINES
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DOI：
10.1099/0022-1317-74-4-685
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发表时间：
1993-04-01
影响因子：
3.8
通讯作者：
MINOR, PD
中科院分区：
文献类型：
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作者：
FERGUSON, M;WOOD, DJ;MINOR, PD
用捕获酶免疫分析法检测了D抗原特异性单克隆抗体与两个生产商的市售脊髓灰质炎疫苗中感染性病毒和灭活病毒的反应性。灭活过程对疫苗中灭活的脊髓灰质炎病毒2型和3型的表位进行了修饰，使得一些与同源感染性病毒株结合的单克隆抗体无法与疫苗中的灭活病毒结合。对于位点1特异性抗体尤其如此。其在小鼠中激发对这些血清型的主要免疫应答。其他表位被部分破坏，导致与感染性病毒相比，单克隆抗体与疫苗中灭活病毒的反应性降低。这些研究表明，疫苗中的灭活病毒在抗原性上与天然病毒不同，因此在体外试验中测量疫苗的效价可能需要仔细选择适当的抗体。此外，福尔马林灭活脊髓灰质炎病毒的接受者产生的一些抗体可能不会有助于保护性免疫应答。
The reactivity of D antigen-specific monoclonal antibodies with infectious virus and inactivated virus in commercial poliovaccines from two manufacturers was examined in capture enzyme immunoassays. Epitopes oil inactivated poliovirus types 2 and 3 in vaccines were modified by the inactivation process to such an extent that some monoclonal antibodies which bound to the homologous infectious virus strain failed to bind to the inactivated virus in the vaccine. This was especially the case for antibodies specific for site 1. which elicits the major immune response to these serotypes in mice. Other epitopes were partially destroyed resulting in a reduction in reactivity of monoclonal antibodies with inactivated virus in vaccines compared to infectious virus. These studies indicate that inactivated virus in vaccine differs antigenically from native virus so that measuring the potency of vaccine in assays in vitro may require careful selection of an appropriate antibody. In addition it is possible that some of the antibodies produced by recipients of formalin-inactivated polioviruses may not contribute to the protective immune response.