Neurospora crassa ro-10 and ro-11 genes encode novel proteins required for nuclear distribution
Neurospora crassa ro-10 and ro-11 genes encode novel proteins required for nuclear distribution
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DOI：
10.1046/j.1365-2958.1999.01421.x
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发表时间：
1999-06-01
影响因子：
3.6
通讯作者：
Plamann, M
中科院分区：
文献类型：
--
作者：
Minke, PF;Lee, IH;Plamann, M
真菌细胞核的运动和分布依赖于细胞质微管和微管相关的运动细胞质动力蛋白。我们已经分离出了数百个粗糙脉孢菌突变体，称为ropy，它们在核分布上有缺陷。ro-1、ro-3和ro-4这三个ro基因已被证明编码细胞质动力蛋白或动力蛋白激活物复合物动力肌动蛋白的亚基。在这份报告中，我们描述了两个额外的ro基因，ro-10和ro-11的分离和初步表征。ro-10和ro-II是分别编码新的24 kDa和75 kDa蛋白质的非必需基因。ro-10和ro-11突变体都保留了产生长的细胞质微管轨道的能力，这表明细胞核分布缺陷不是由微管细胞骨架中的总缺陷引起的。RO 10以及RO 4（肌动蛋白相关蛋白ARP 1，动力肌动蛋白中最丰富的亚基）似乎是RO 3（p150（Glued））（动力肌动蛋白中最大的亚基）稳定性所必需的。我们建议，RO-10突变体缺乏适当的核分布，因为RO 10是dynactin的亚基和dynactin活性所需的或必不可少的dynactin复合物的组装。ro-ll突变对RO 1或RO 3水平没有影响，对细胞质动力蛋白和动力肌动蛋白的定位模式只有很小的影响。厚菌丝仍然未知。
Movement and distribution of nuclei in fungi have been shown to be dependent on cytoplasmic microtubules and the microtubule-associated motor cytoplasmic dynein. We have isolated hundreds of Neurospora crassa mutants, known as ropy, that are defective in nuclear distribution. Three of the ro genes, ro-1, ro-3 and ro-4, have been shown to encode subunits of either cytoplasmic dynein or the dynein activator complex, dynactin. In this report, we describe the isolation and initial characterization of two additional ro genes, ro-10 and ro-ll. ro-10 and ro-ll are non-essential genes that encode novel 24 kDa and 75 kDa proteins respectively. Both ro-10 and ro-ll mutants retain the ability to generate long cytoplasmic microtubule tracks, suggesting that the nuclear distribution defect is not caused by a gross defect in the microtubule cytoskeleton. RO10, as well as RO4 (actin-related protein ARP1, the most abundant subunit of dynactin), appears to be required for the stability of RO3 (p150(Glued)), the largest subunit of dynactin. We propose that ro-10 mutants lack proper nuclear distribution, because RO10 is either a subunit of dynactin and required for dynactin activity or essential for assembly of the dynactin complex. ro-ll mutations have no effect on RO1 or RO3 levels and have only a very slight effect on the localization pattern of cytoplasmic dynein and dynactin, The role of RO11 in the movement and distribution of nuclei in N. crassa hyphae remains unknown.