A novel "cleaved prolactin" in the rat pituitary: Part II. In vivo mammary mitogenic activity of its N-terminal 16K moiety.
A novel "cleaved prolactin" in the rat pituitary: Part II. In vivo mammary mitogenic activity of its N-terminal 16K moiety.
复制标题
大鼠垂体中的新型“裂解催乳素”：第二部分。
DOI：
10.1016/s0006-291x(80)80102-1
复制
发表时间：
1980
影响因子：
3.1
通讯作者：
I. Mittra
中科院分区：
文献类型：
--
作者：
I. Mittra
在还原条件下，使用新鲜大鼠垂体提取物或培养基的制备型十二烷基硫酸钠聚丙烯酰胺凝胶电泳将完整的催乳素与切割的催乳素的16K和8K部分分离。从未染色的凝胶中切下含有完整催乳素和16K条带的凝胶区域，并将其作为单一接种物皮下注射到成年处女雌性大鼠中。 16K多肽显着增加了乳腺上皮细胞中DNA合成和细胞分裂的速率。含有完整催乳素的凝胶带无效。在单独的实验中，以生理剂量注射 NIH 大鼠催乳素 B-1 生理盐水也未能提高乳腺细胞分裂速度。
Preparative sodium dodecyl sulphate polyacrylamide gel electrophoresis of fresh rat pituitary extract or culture medium, under reducing conditions, was used to separate intact prolactin from the 16K and 8K moieties of cleaved prolactin. Regions of the gel containing the intact prolactin and 16K bands were excised from unstained gels and injected subcutaneously into adult virgin female rats as a single inoculum. The 16K polypeptide significantly increased the rate of DNA synthesis and cell division in the mammary epithelial cells. Gel bands containing intact prolactin were ineffective. In separate experiments, the injection of NIH rat prolactin B-1 in saline, given in physiological doses, also failed to increase the rate of mammary cell division.