Visualization of hypoxia in microscopic tumors by immunofluorescent microscopy
Visualization of hypoxia in microscopic tumors by immunofluorescent microscopy
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DOI：
10.1158/0008-5472.can-06-4353
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发表时间：
2007-08-15
期刊：
影响因子：
11.2
通讯作者：
O'Donoghue, Joseph A.
中科院分区：
文献类型：
--
作者：
Li, Xiao-Feng;Carlin, Sean;O'Donoghue, Joseph A.
肿瘤缺氧常见于原发性实体恶性肿瘤，但亚临床微转移性疾病的缺氧状态在很大程度上是未知的。在弥散性腹膜疾病动物模型和皮内生长肿瘤动物模型中研究了显微肿瘤缺氧的分布。来源于人类结直肠癌细胞系HT29和HCT-8的肿瘤直径从几百微米到几毫米不等。用免疫荧光法检测吡咪唑和低氧调节蛋白碳酸酐酶9的缺氧情况。分别用Hoechst 33342、溴脱氧尿苷和CD31染色观察肿瘤血液灌注、细胞增殖和血管分布。一般来说，肿瘤
Tumor hypoxia is commonly observed in primary solid malignancies but the hypoxic status of subclinical micro-metastatic disease is largely unknown. The distribution of hypoxia in microscopic tumors was studied in animal models of disseminated peritoneal disease and intradermal (i.d.) growing tumors. Tumors derived from human colorectal adenocarcinoma cell lines HT29 and HCT-8 ranged in size from a few hundred microns to several millimeters in diameter. Hypoxia was detected by immunofluorescent visualization of pimonidazole and the hypoxia-regulated protein carbonic anhydrase 9. Tumor blood perfusion, cellular proliferation, and vascularity were visualized using Hoechst 33342, bromodeoxyuridine, and CD31 staining, respectively. In general, tumors of