A comparative study of insertion/deletion polymorphisms applied among Southwest, South and Northwest Chinese populations using Investigator (R) DIPplex
A comparative study of insertion/deletion polymorphisms applied among Southwest, South and Northwest Chinese populations using Investigator (R) DIPplex
复制标题
使用 Investigator (R) DIPplex 对中国西南、华南和西北人群插入/缺失多态性进行比较研究
DOI：
10.1016/j.fsigen.2015.08.005
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发表时间：
2016
影响因子：
3.1
通讯作者：
Zhang Lin
中科院分区：
文献类型：
--
作者：
Wang Li;Lv Meili;Zaumsegel Daniel;Zhang Lushun;Liu Fengji;Xiang Jihua;Li Jun;Schneider Peter M.;Liang Weibo;Zhang Lin
双等位基因插入/缺失多态性（INDEL）的低突变率、短扩增子大小和长度变化特征是允许将STR和SNP标记两者的优点组合用于遗传分析的特征。Qiagen Investigator DIPplex试剂盒包含30个双等位基因常染色体INDEL加釉原蛋白，用于法医个人鉴定和亲子鉴定。本研究采用DIPplex试剂盒对中国西南、华南和西北三个地区的汉族、藏族、彝族、壮族、侗族、苗族和维吾尔族7个民族的一般行为、法医学和人口学资料进行了比较研究。经Bonferroni校正后，所有供试群体的30个标记均未偏离Hardy-Weinberg平衡（HWE）。组合匹配概率的范围从侗族的1.4273 × 10 - 10到维吾尔族的1.2817 × 10 - 12。亲子关系排除的综合把握度为0.9854 ~ 0.9968。7个民族间的成对遗传差异（FST）均小于0.05，STRUCTURE分析的结果表明，这3个地区的群体间不存在遗传相似性的亚结构。DIPplex试剂盒可作为这三个地区法医学个人身份鉴定的辅助工具。
The low mutation rates, short amplicon sizes and length variation characteristics of biallelic insertion/deletion polymorphisms (INDELs) are features that allow combining advantages of both STR and SNP markers for genetic analysis. The Qiagen Investigator DIPplex kit contains 30 biallelic autosomal INDELs plus Amelogenin for forensic personal identification and parentage testing. This study compares general performances, forensic and population data collected in 7 Chinese ethnic populations (Han, Tibetan, Yi, Zhuang, Dong, Miao and Uyghur) from three areas (Southwest, South and Northwest) of China by DIPplex kit. There were no significant departures from Hardy–Weinberg equilibrium (HWE) of the 30 markers in all the tested populations after Bonferroni correction. The combined matching probabilities ranged from 1.4273 × 10−10for the Dong minority to 1.2817 × 10−12for the Uyghur minority. The combined power of paternity exclusion was from 0.9854 to 0.9968. All pairwise genetic differences (FST) between the seven ethnic groups were less than 0.05, and the results of STRUCTURE analysis did not indicate the presence of substructures suggesting genetic similarity among the populations in these three areas. The DIPplex kit can be used as a good additional tool for forensic personal identification in these three areas of China.