A new method for the investigation of capillary structure
A new method for the investigation of capillary structure
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DOI：
10.1016/s0165-0270(01)00494-0
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发表时间：
2002-01-30
影响因子：
3
通讯作者：
Anderson, BJ
中科院分区：
文献类型：
--
作者：
Tata, DA;Anderson, BJ
许多生理条件以及行为条件已被证明会影响中枢神经系统血管结构。许多用于研究这些结构改变的方法利用了灌注到脉管系统中的粘性物质（例如明胶中的印度墨水）的可见性。然而，溶液的高粘度可能导致血管灌注不完全。本研究的目的是测试是否毛细血管与固定剂灌注的组织中看到的，包埋在火棉胶和亚甲基蓝-天青II染色（n = 6）可能是一个有用的替代调查脑血管结构。该方法进行了比较，从六只大鼠的组织灌注印度墨水在明胶和甲酚紫染色。在火棉胶包埋的标准灌注组织中的定性血管产生了具有染色的周细胞的透明血管。两种方法在分支点与细胞的比率、单个毛细血管的长度、每毫米血管长度（3）和毛细血管迂曲度方面没有差异。火棉胶包埋组织中的毛细血管直径大于印度墨水灌注组织。测量血管壁之间的直径似乎提供了比印度油墨颜料之间的最宽距离更准确的测量。定量比较表明，用标准固定剂灌注，然后包埋在火棉胶中，可提供与印度墨水灌注组织相当的血管定量。本方法具有几个优点，包括周细胞的可视化，增加完全灌注的可能性，否则可能被不透明的血管遮蔽的细胞的清晰视图，以及使用备用大脑半球用于血管超微结构研究的可能性。(C)2002 Elsevier Science B. V.保留所有权利。
Numerous physiological conditions as well as behavioral conditions have been shown to influence central nervous system vascular structure. Many of the methods used to investigate these structural alterations take advantage of the visibility of viscous substances (e.g. India ink in gelatin) perfused into the vasculature. The high viscosity of the solution, however, can cause incomplete vessel perfusion. The aim of the present study was to test whether or not capillaries seen in tissue perfused with fixative, embedded in celloidin and stained with Methylene Blue-Azure II (n = 6) could be a useful alternative for the investigation of brain vascular structure. The method was compared to tissue from six rats perfused with India ink in gelatin and stained with cresyl violet. Qualitatively, vessels in the standard perfused tissue embedded in celloidin yielded clear vessels with stained pericytes. The two methods did not differ in branch point to cell ratio, length of individual capillaries, vessel length per mm(3), and capillary tortuosity. The capillary diameter was greater in the celloidin embedded tissue than in the India ink perfused tissue. Measuring the diameter between vessel walls appears to provide a more accurate measure than the widest distance between India ink pigments. Quantitative comparisons suggest that perfusion with standard fixative followed by embedding in celloidin provides vascular quantification comparable to that from India ink perfused tissue. The present method has several advantages, which include visualization of pericytes, increased probability of complete perfusion, clear view of cells that might otherwise be obscured by opaque vessels, and the possibility of using the alternate cerebral hemisphere for investigation of vascular ultrastructure. (C) 2002 Elsevier Science B.V. All rights reserved.