COMPARATIVE LABELING OF RAT EPIDIDYMAL SPERMATOZOA BY INTRATESTICULARLY ADMINISTERED (ZNCL2)-ZN-65 AND [CYSTEINE-S-35
COMPARATIVE LABELING OF RAT EPIDIDYMAL SPERMATOZOA BY INTRATESTICULARLY ADMINISTERED (ZNCL2)-ZN-65 AND [CYSTEINE-S-35
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DOI：
10.1530/jrf.0.0610065
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
CALVIN, HI
中科院分区：
文献类型：
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作者：
CALVIN, HI
分别于注射后2天、6天、10天、14天、18天、22天和28天，分别从附睾头、尾部采集注射Ci~(65)ZnCl2 20微米和Ci[35S]10微米的精子。头精子中各同位素的比活力在第10天达到最高，尾部精子在第35s和18d达到最高水平，~(65)Zn在第18天达到最高水平。通过计算~(65)Zn/~(35)S比值来估算~(65)Zn和~(35)S的相对行为，可得出以下结论。与精子相关的35S比65Zn稍早到达附睾，并可能更容易被结合到很晚的精子细胞的蛋白质中。假设总保留时间为35s，在从头部到尾部的运输过程中，约60%的~(65)Zn从精子和75%的分离精子头部丢失。生精上皮对~(65)Zn的保留优于对[~(35)S]半胱氨酸的保留，这从附睾头精子和附睾尾精子比活度达到峰值后，~(65)Zn/~(35)S比值的升高来说明。在分离的精子头部中，只有一小部分的同位素被回收，这表明标记的主要位置在精子尾部。在注射后2至10天，睾丸匀浆各部分中的65Zn/35S比值增加，证实了~(65)Zn在睾丸中的良好保留。在此期间，这两种同位素似乎都从睾丸胞浆转移到颗粒物质中。
Spermatozoa of rats injected intratesticularly with 20 .mu.Ci 65ZnCl2 and 10 .mu.Ci [35S]cysteine were collected from the caput and cauda of the epididymis at 2, 6, 10, 14, 18, 22 and 28 days after injection. The highest specific activities for each isotope were observed in spermatozoa from the caput on day 10. Maximal levels in spermatozoa from the cauda were obtained on days 14 and 18 for 35S and day 18 for 65Zn. Estimation of the relative behavior of 65Zn and 35S by calculation of 65Zn/35S ratios suggested the following items. 35S associated with spermatozoa arrived in the epididymis slightly in advance of 65Zn and was probably incorporated more readily into proteins of very late spermatids. About 60% of 65Zn was lost from spermatozoa and 75% from isolated sperm heads during transit from caput to cauda, assuming total retention of 35S. Retention of 65Zn by the seminiferous epithelium was superior to that of [35S]cysteine, as indicated by increasing 65Zn/35S ratios following the days of peak-specific activity in both caput and cauda epididymidal spermatozoa. Only small percentages of either isotope were recovered in isolated sperm heads indicating that the primary sites of labeling were in the sperm tail. Superior retention of 65Zn by testis was confirmed by increasing 65Zn/35S ratios in individual fractions of testicular homogenates between 2 and 10 days after injection. Both isotopes appeared to be transferred from the testis cytosol to particulate material during this period.