Responses of Brassica oleracea cultivars to infestation by the aphid Brevicoryne brassicae:: an ecological and molecular approach
Responses of Brassica oleracea cultivars to infestation by the aphid Brevicoryne brassicae:: an ecological and molecular approach
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DOI：
10.1111/j.1365-3040.2008.01871.x
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发表时间：
2008-11-01
影响因子：
7.3
通讯作者：
Vosman, Ben
中科院分区：
文献类型：
--
作者：
Broekgaarden, Colette;Poelman, Erik H.;Vosman, Ben
对植食性昆虫的抗性或感病性的种内变异已经通过生物测定被广泛研究。然而，很少有研究将其与完整的转录组学分析相结合。本文采用这种方法研究了甘蓝蚜与四种白色甘蓝（Brassica oleracea var. capitata）栽培品种。无论是在温室和田间条件下，两个品种明显支持更快的蚜虫种群发展比其他两个，表明蚜虫种群发展在很大程度上是独立的环境条件。基于拟南芥基因组的70-mer寡核苷酸微阵列的全基因组转录组学分析表明，只有少数基因受到差异调节，并且这种调节具有高度的品种特异性。两个B表达行为的时间模式。在所有四个品种的芸苔属响应基因，以及有针对性的研究，采用A。拟南芥敲除突变体揭示了胰蛋白酶和蛋白酶抑制剂在防御B中的可能作用。芸苔属。相反，木葡聚糖内切转葡糖基酶似乎对蚜虫的表现没有影响。总体而言，这项研究表明，在B明显的种内变异。B.在温室和田间条件下的甘蓝品种，可以部分解释诱导的转录变化的某些差异。
Intraspecific variation in resistance or susceptibility to herbivorous insects has been widely studied through bioassays. However, few studies have combined this with a full transcriptomic analysis. Here, we take such an approach to study the interaction between the aphid Brevicoryne brassicae and four white cabbage (Brassica oleracea var. capitata) cultivars. Both under glasshouse and field conditions, two of the cultivars clearly supported a faster aphid population development than the other two, indicating that aphid population development was largely independent of the environmental conditions. Genome-wide transcriptomic analysis using 70-mer oligonucleotide microarrays based on the Arabidopsis thaliana genome showed that only a small number of genes were differentially regulated, and that this regulation was highly cultivar specific. The temporal pattern in the expression behaviour of two B. brassicae-responsive genes in all four cultivars together with targeted studies employing A. thaliana knockout mutants revealed a possible role for a trypsin-and-protease inhibitor in defence against B. brassicae. Conversely, a xyloglucan endotransglucosylase seemed to have no effect on aphid performance. Overall, this study shows clear intraspecific variation in B. brassicae susceptibility among B. oleracea cultivars under glasshouse and field conditions that can be partly explained by certain differences in induced transcriptional changes.