A GENETIC-ANALYSIS OF MIXED POPULATION IN THEILERIA-SERGENTI STOCKS AND ISOLATES USING ALLELE-SPECIFIC POLYMERASE CHAIN-REACTION
A GENETIC-ANALYSIS OF MIXED POPULATION IN THEILERIA-SERGENTI STOCKS AND ISOLATES USING ALLELE-SPECIFIC POLYMERASE CHAIN-REACTION
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DOI：
10.1292/jvms.57.279
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发表时间：
1995-04-01
影响因子：
1.2
通讯作者：
ONUMA, M
中科院分区：
文献类型：
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作者：
KUBOTA, S;SUGIMOTO, C;ONUMA, M
为了区分携带两种等位基因形式p33/32（瑟氏泰勒虫的免疫显性梨质表面蛋白）的寄生虫种群，设计了两组寡核苷酸引物，通过聚合酶链反应（PCR）扩增两种等位基因中的任一种。每组引物差异扩增Ikeda型或Chitose型p33/32基因。通过使用该等位基因特异性PCR和扩增产物的限制性内切酶消化，对从日本不同地理区域收集的田间分离株内的寄生虫种群进行了分析。用PCR方法检测了20个分离株和20个原种中的13个样品，发现了两种等位基因。其余7例为池田型或甲壳糖型p33/32等位基因。这些结果表明，大多数T.在日本，感染sergentii的小牛（11/15）携带至少两种不同的p33/32等位基因的混合寄生虫种群。
In order to differentiate parasite populations bearing two allelic forms of p33/32, an immunodominant piroplasm surface protein of Theileria sergenti, two sets of oligonucleotide primers were designed to amplify either of the two allele by the polymerase chain reaction (PCR). Each set of the primers differentially amplified either of Ikeda- or Chitose-type p33/32 genes. By using this allele-specific PCR and restriction enzyme digestion of amplified products, parasite populations within field isolates collected from different geographical regions in Japan were analyzed. Both of the allelic forms were detected in 13 samples out of 20 isolates and stocks by PCR. Either of Ikeda or Chitose type of p33/32 allele was detected in the other 7 samples. These results indicated that the majority of T. sergenti-infected calves (11/15) in Japan harbored mixed parasite populations bearing at least two different alleles of p33/32.