Improved screening procedure for biogenic amine production by lactic acid bacteria
Improved screening procedure for biogenic amine production by lactic acid bacteria
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DOI：
10.1016/s0168-1605(99)00152-x
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发表时间：
1999-12-01
影响因子：
5.4
通讯作者：
Holzapfel, WH
中科院分区：
文献类型：
--
作者：
Bover-Cid, S;Holzapfel, WH
建立了一种改进的检测氨基酸脱羧酶阳性微生物（特别是乳酸菌）的平板筛选法。通过采用HPLC方法确认胺形成能力，定量评价了设计培养基的适用性和检测水平。研究了不同来源的乳酸菌（LAB）产生生物胺（BA）酪胺、组胺、腐胺和尸胺的潜力，包括发酵剂培养物、保护性培养物、模式菌株和从不同食品中分离的菌株。此外，还检查了几种肠杆菌科菌株。对先前描述的方法的修改包括降低葡萄糖和氯化钠浓度，以及用碳酸钙和磷酸钾增加缓冲效果。此外，吡哆醛-5-磷酸被包括作为辅脱羧酶因子，其增强作用的氨基酸脱羧酶活性。筛选平板法与化学分析结果具有良好的相关性，且方法简单，可作为一种灵敏、适用于研究乳酸菌产生物胺能力的方法。酪胺是所研究的LAB菌株形成的主要胺。肠球菌属、肉杆菌属和一些乳杆菌属菌株，特别是Lb。curvatus，Lb. brevis和Lb. Buchneri是最强的酪胺形成剂。几种乳酸杆菌菌株，明串珠菌属，魏氏菌属而片球菌没有显示出任何产生胺的潜力。肠杆菌科与尸胺和腐胺的形成有关。任何供试菌株均未检测到显著的组胺产生。(C)1999 Elsevier Science B;V.保留所有权利。
An improved screening plate method for the detection of amino acid decarboxylase-positive microorganisms (especially lactic acid bacteria) was developed. The suitability and detection level of the designed medium were quantitatively evaluated by confirmation of amine-forming capacity using an HPLC procedure. The potential to produce the biogenic amines (BA) tyramine, histamine, putrescine, and cadaverine, was investigated in a wide number of lactic acid bacteria (LAB) of different origin, including starter cultures, protective cultures, type strains and strains isolated from different food products. Also, several strains of Enterobacteriaceae were examined. Modifications to previously described methods included lowering glucose and sodium chloride concentrations, and increasing the buffer effect with calcium carbonate and potassium phosphate. In addition, pyridoxal-5-phosphate was included as a codecarboxylase factor for its enhancing effect on the amino acid decarboxylase activity. The screening plate method showed a good correlation with the chemical analysis and due to its simplicity it is presented as a suitable and sensitive method to investigate the capacity of biogenic amine production by LAB. Tyramine was the: main amine formed by the LAB strains investigated. Enterococci, carnobacteria and some strains of lactobacilli, particularly of Lb. curvatus, Lb. brevis and Lb. buchneri, were the most intensive tyramine formers. Several strains of lactobacilli, Leuconostoc spp., Weissella spp. and pediococci did not show any potential to produce amines. Enterobacteriaceae were associated with cadaverine and putrescine formation. No significant histamine production could be detected for any of the strains tested. (C) 1999 Elsevier Science B;V. All rights reserved.