Proto-oncogene expression during two-stage carcinogenesis in mouse skin.
Proto-oncogene expression during two-stage carcinogenesis in mouse skin.
复制标题
小鼠皮肤两阶段癌变过程中原癌基因的表达。
DOI：
10.1093/carcin/6.4.655
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发表时间：
1985
期刊：
影响因子：
4.7
通讯作者：
Yuspa,SH
中科院分区：
文献类型：
--
作者：
Toftgard,R;Roop,DR;Yuspa,SH
在化学诱导的肿瘤中、在小鼠皮肤中和在用肿瘤促进剂处理后在小鼠表皮中测定了与同源基因rasHa、rasKi、fos、myc、ablandraf的细胞同源物的表达水平。未发现上述任何原癌基因的表达显著增加。在乳头状瘤和癌中以及重复应用12-O-十四烷酰佛波醇-13-乙酸酯（TPA）后，表达均降低。体外用TPA处理原代小鼠表皮细胞，不能诱导原癌基因表达的任何显著改变。用化学致癌物处理表皮细胞得到的细胞系表现出与未处理的原代表皮基底细胞相似的表达水平。这些结果表明，在小鼠皮肤中的肿瘤诱导不需要增加所研究的原癌基因的表达。在肿瘤中和反复应用TPA后，表达水平较低，这可能表明该基因在表皮增殖或分化的某些方面起作用。
The level of expression of the cellular homologues to the on cogenesrasHa,rasKi,fos, myc, ablandrafwas determined in chemically induced tumors, in mouse skin and in mouse epidermis after treatment with a tumor promoter. No significant increase in expression was found for any of the above proto-oncogenes. The expression ofablwas reduced in both papillomas and carcinomas and after repeated applications of 12-O-tetradecanoylphorbol-13-acetate (TPA). Treatment of primary mouse epidermal cells with TPAin vitrofailed to induce any marked alterations in proto-oncogene expression. Cell lines derived by treatment of epidermal cells with chemical carcinogens showed expression levels similar to untreated primary epidermal basal cells. These results suggest that increased expression of the proto-oncogenes under study is not required for tumor induction in mouse skin. The lower level of expression ofablin tumors and after repeated applications of TPA may indicate a role for this gene in some aspect of epidermal proliferation or differentiation.