ASTROGLIAL AND AXONAL PROTEINS IN ISOLATED BRAIN FILAMENTS .1. ISOLATION OF THE GLIAL FIBRILLARY ACIDIC PROTEIN AND OF AN IMMUNOLOGICALLY ACTIVE CYANOGEN-BROMIDE PEPTIDE FROM BRAIN FILAMENT PREPARATIONS OF BOVINE WHITE MATTER
ASTROGLIAL AND AXONAL PROTEINS IN ISOLATED BRAIN FILAMENTS .1. ISOLATION OF THE GLIAL FIBRILLARY ACIDIC PROTEIN AND OF AN IMMUNOLOGICALLY ACTIVE CYANOGEN-BROMIDE PEPTIDE FROM BRAIN FILAMENT PREPARATIONS OF BOVINE WHITE MATTER
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DOI：
10.1016/0005-2795(79)90161-2
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
BIGNAMI, A
中科院分区：
文献类型：
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作者：
DAHL, D;BIGNAMI, A
根据诺顿程序，通过免疫亲和层析从从牛白质分离的脑丝制剂的8M尿素提取物中纯化神经胶质原纤维酸性蛋白和免疫活性溴化氰肽。该蛋白质占.apprx。该制剂中总蛋白质的 30%，并且是 50,000 MW 范围内的最大部分。未吸收至免疫琼脂糖柱的部分通过双重免疫扩散与神经丝抗血清反应。在十二烷基硫酸钠凝胶电泳上，未吸附级分中的主要条带位于 74,000 道尔顿和 100,000 以上。在 50,000 MW 范围内看到了几个波段。胶质细胞和神经丝在诺顿的程序中共同纯化，并且神经丝在多肽组成上可能是异质的。
The glial fibrillary acidic protein and an immunologically active cyanogen bromide peptide were purified by immunoaffinity chromatography from 8 M urea extracts of brain filament preparations isolated from bovine white matter according to Norton''s procedure. The protein accounted for .apprx. 30% of the total protein in this preparation and for the largest fraction in the 50,000 MW range. The fraction not absorbed to the immuno-Sepharose column reacted with neurofilament antisera by double immunodiffusion. On sodium dodecyl sulfate gel electrophoresis the main bands in the non-adsorbed fraction were at 74,000 daltons and above 100,000. Several bands were seen in the 50,000 MW range. Glio-and neurofilaments co-purify together in Norton''s procedure and neurofilaments are probably heterogeneous in polypeptide composition.