Latrunculin A Dramatically Improves the Developmental Capacity of Nuclear Transfer Embryos Derived from Gene-Modified Clawn Miniature Pig Cells
Latrunculin A Dramatically Improves the Developmental Capacity of Nuclear Transfer Embryos Derived from Gene-Modified Clawn Miniature Pig Cells
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DOI：
10.1089/cell.2009.0066
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发表时间：
2010-04-01
影响因子：
1.6
通讯作者：
Yoshida, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Himaki, Takehiro;Mori, Hironori;Yoshida, Mitsutoshi
本研究旨在研究肌动蛋白聚合抑制剂latrunculin A （LatA）活化后处理对源自基因修饰的微型猪体细胞核移植（SCNT）胚胎体外和体内发育的影响。融合和激活后，SCNT胚胎分别用或不加细胞骨架抑制剂[LatA或10.4 μ M细胞松弛素B (CB)]处理2h。0.5 μ M LatA处理的胚胎的卵裂率显著高于未添加细胞骨架抑制剂的CB处理的胚胎（p < 0.05）。此外，0.5和1 μ M LatA处理的胚胎囊胚形成率显著高于未添加细胞骨架抑制剂的CB处理的胚胎囊胚形成率（p < 0.05）。此外，0.5 mM LatA处理后的胚胎移植在受体子宫中获得5个胎儿。本研究结果首次表明，经LatA后激活处理可有效提高基因修饰克隆微型猪胚胎的体外发育能力，且经LatA处理的胚胎具有发育成胎儿的能力。
This study was carried out to examine the effect of postactivation treatment with latrunculin A (LatA), an actin polymerisation inhibitor, on in vitro and in vivo development of somatic cell nuclear transfer (SCNT) embryos derived from gene-modified Clawn miniature pig cells. After the fusion and activation, SCNT embryos were treated with or without a cytoskeletal inhibitor [LatA or 10.4 mu M cytochalasin B (CB) for 2h]. The cleavage rate was significantly higher (p < 0.05) in embryos exposed to 0.5 mu M LatA than those in embryos exposed to CB and without a cytoskeletal inhibitor. Moreover, the blastocyst formation rate was significantly higher (p < 0.05) in embryos exposed to 0.5 or 1 mu M LatA than those in embryos exposed to CB and without a cytoskeletal inhibitor. In addition, five fetuses were obtained from recipient uteri after transfer of embryos treated with 0.5 mM LatA. The results of this study show for the first time that postactivation treatment with LatA is effective to improve in vitro developmental capacity of gene-modified cloned miniature pig embryos and embryos treated with LatA have the ability to develop into fetuses.