The expression and significance of five types of miRNAs in breast cancer.
The expression and significance of five types of miRNAs in breast cancer.
复制标题
DOI：
10.12659/msmbr.891246
复制
发表时间：
2014-07-21
影响因子：
2.8
通讯作者：
Ma Q
中科院分区：
文献类型：
--
作者：
Min W;Wang B;Li J;Han J;Zhao Y;Su W;Dai Z;Wang X;Ma Q
本研究旨在探讨5类mirna在乳腺癌中的表达及其意义，为利用这些mirna进行乳腺癌的诊断和治疗，从而提高医疗服务水平提供理论和实践基础。采用茎环实时RT-PCR检测35例乳腺癌及邻近正常乳腺组织中miR-145、miR-21、miR-10b、miR-125a、miR-206的表达水平，分析miRNAs表达与乳腺癌临床病理特征的关系。免疫组化法检测小鼠雌激素受体（ER）和孕激素受体（PR）的表达水平。采用荧光原位杂交法检测HER-2和TOP 2A。miR-145、miR-125a、miR-206在乳腺癌组织中的表达水平低于癌旁正常组织。MiR-145与肿瘤大小、淋巴结转移、ER、HER-2、TOP 2A呈负相关（P<0.05），与年龄、月经、PR无关。MiR-125a与淋巴结状态、HER-2状态呈负相关（P<0.05），而肿瘤大小、年龄、月经、ER、PR为独立因素。MiR-206表达与ER状态阴性、PR状态阴性、HER-2状态阴性相关（P<0.05），与年龄、月经、淋巴结转移、TOP 2A无关。MiR-21、miR-10b在乳腺癌组织中的表达明显高于癌旁组织（P<0.05）。MiR-21在经后淋巴结转移患者中高表达（P<0.05），且与肿瘤大小、ER、PR、TOP 2A表达无关。MiR-10b表达与乳腺癌肿瘤大小、淋巴结转移、TOP 2A状态呈正相关（P<0.05），而与年龄、月经、ER、PR、HER-2无相关性。MiR-145、miR-21、miR-10b、miR-125a和miR-206可能在乳腺癌的发展和侵袭中发挥重要作用。
This study aimed to investigate the expression and significance of 5 types of miRNAs in breast cancer to provide a theoretical and practical foundation for using these miRNAs in the diagnosis and treatment of breast cancer, thereby improving medical services. Stem-loop real-time RT-PCR was used to detect the expression levels of miR-145, miR-21, miR-10b, miR-125a, and miR-206 in 35 cases of breast cancer and adjacent normal breast tissues, and to analyze the relationship of miRNAs expression with clinicopathological features of breast cancer. The expression levels of estrogen receptor (ER) and progesterone receptor (PR) were examined by immunohistochemistry. Fluorescence in situ hybridization was used for the detection of HER-2 and TOP 2A. The expression levels of miR-145, miR-125a, and miR-206 in breast cancer were lower than those in adjacent normal tissues. MiR-145 was negatively correlated with tumor size, lymph node metastasis, ER, HER-2, and TOP 2A (P<0.05), regardless of age, menstruation, and PR. MiR-125a was correlated with negative node status, negative HER-2 status (P<0.05), whereas tumor size, age, menstruation, ER, and PR were independent factors. MiR-206 expression was correlated with negative ER status, negative PR status, and negative HER-2 status (P<0.05), regardless of age, menstruation, lymph node metastasis, and TOP 2A. MiR-21 and miR-10b expression in breast cancer tissues was significantly higher than that in adjacent tissues (P<0.05). MiR-21 in post-menstrual patients with lymph node metastasis was highly expressed (P<0.05), and had no correlations with tumor size, ER, PR, and TOP 2A expression. MiR-10b expression was positively correlated with breast cancer tumor size, lymph node metastasis, and TOP 2A status (P<0.05), but had no correlations with age, menstruation, ER, PR, and HER-2. MiR-145, miR-21, miR-10b, miR-125a, and miR-206 may play important roles in breast cancer development and invasion.